Standard Name	PRK1 (see Nomenclature conflict Note)
Systematic Name	YIL095W
Alias	PAK1
Feature Type	ORF, Verified
Description	Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication (1, 2)
Name Description	p53 Regulatory Kinase 3

Chromosomal Location	ChrIX:183937 to 186369 | ORF Map | GBrowse

	View Computational GO annotations for PRK1
Molecular Function
Manually curated	protein serine/threonine kinase activity (IDA)
High-throughput	protein kinase activity (IDA)
Biological Process
Manually curated	actin cortical patch assembly (TAS) actin filament organization (IMP) cytokinesis (TAS) protein phosphorylation (IDA) regulation of endocytosis (IMP)
High-throughput	protein phosphorylation (IDA)
Cellular Component
Manually curated	actin cortical patch (IDA)
High-throughput	mating projection tip (IDA)

Classical genetics
null	chronological lifespan: decreased Scd5p modification: decreased Pan1p modification: decreased resistance to sirolimus: increased resistance to doxorubicin: decreased
overexpression	resistance to doxorubicin: increased
Large-scale survey
null	polyphosphate accumulation: abnormal competitive fitness: decreased resistance to cycloheximide: increased resistance to bleomycin: increased resistance to caffeine: increased resistance to hydroxyurea: increased resistance to mycophenolic acid: increased resistance to camptothecin: decreased respiratory growth: decreased rate stress resistance: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	200 total interaction(s) for 148 unique genes/features.
Physical Interactions	Affinity Capture-MS: 25 Affinity Capture-RNA: 2 Affinity Capture-Western: 7 Biochemical Activity: 69 PCA: 1 Protein-peptide: 1 Reconstituted Complex: 1 Two-hybrid: 9
Genetic Interactions	Dosage Growth Defect: 13 Dosage Lethality: 1 Negative Genetic: 42 Phenotypic Enhancement: 4 Phenotypic Suppression: 3 Positive Genetic: 6 Synthetic Growth Defect: 5 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 5 Synthetic Rescue: 5
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIX:183937 to 186369 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2433 183937..186369 2011-02-03 1994-12-10
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001357