SDS3/YIL084C Summary

SDS3/YIL084C Summary

Standard Name	SDS3 1
Systematic Name	YIL084C
Feature Type	ORF, Verified
Description	Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes (2, 3, 4)
Name Description	Suppressor of Defective Silencing 5

Chromosomal Location	ChrIX:203259 to 202276 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All SDS3 GO evidence and references

	View Computational GO annotations for SDS3
Molecular Function
Manually curated	contributes_to histone deacetylase activity (IMP)
Biological Process
Manually curated	chromatin silencing (IGI, IMP) histone deacetylation (IMP, IPI) negative regulation of chromatin silencing at rDNA (IMP) negative regulation of chromatin silencing at silent mating-type cassette (IMP) negative regulation of chromatin silencing at telomere (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription from RNA polymerase II promoter in response to heat stress (IMP)
Cellular Component
Manually curated	Rpd3L complex (IDA)
High-throughput	Rpd3L complex (IDA) Rpd3L-Expanded complex (IDA)

Mutant phenotypes All SDS3 Phenotype evidence and references

Classical genetics
null	chronological lifespan: decreased prion formation: decreased resistance to tellurite: decreased resistance to camptothecin: decreased
Large-scale survey
null	acid pH resistance: decreased polyphosphate accumulation: abnormal competitive fitness: decreased dessication resistance: decreased endocytosis: decreased haploproficient resistance to cycloheximide: decreased resistance to sodium arsenite: decreased resistance to tunicamycin: decreased resistance to 1,4-dithiothreitol: decreased sporulation: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SDS3 Interaction evidence and references

	558 total interaction(s) for 322 unique genes/features.
Physical Interactions	Affinity Capture-MS: 54 Affinity Capture-Western: 10 Biochemical Activity: 3 Co-purification: 1 Reconstituted Complex: 1 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 4 Negative Genetic: 345 Phenotypic Suppression: 4 Positive Genetic: 74 Synthetic Growth Defect: 35 Synthetic Lethality: 14 Synthetic Rescue: 10
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SDS3 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIX:203259 to 202276 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..984	203259..202276	2011-02-03	1994-12-10

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001346

References cited on this page View Complete Literature Guide for SDS3

1)	Sussel L, et al. (1995) Suppressors of defective silencing in yeast: effects on transcriptional repression at the HMR locus, cell growth and telomere structure. Genetics 141(3):873-88
2)	Vannier D, et al. (1996) Evidence that the transcriptional regulators SIN3 and RPD3, and a novel gene (SDS3) with similar functions, are involved in transcriptional silencing in S. cerevisiae. Genetics 144(4):1343-53
3)	Vannier D, et al. (2001) A role for Sds3p, a component of the Rpd3p/Sin3p deacetylase complex, in maintaining cellular integrity in Saccharomyces cerevisiae. Mol Genet Genomics 265(3):560-8
4)	Lechner T, et al. (2000) Sds3 (suppressor of defective silencing 3) is an integral component of the yeast Sin3[middle dot]Rpd3 histone deacetylase complex and is required for histone deacetylase activity. J Biol Chem 275(52):40961-6
5)	Sussel L, et al. (1993) Epigenetic switching of transcriptional states: cis- and trans-acting factors affecting establishment of silencing at the HMR locus in Saccharomyces cerevisiae. Mol Cell Biol 13(7):3919-28