Standard Name	SEC28
Systematic Name	YIL076W
Alias	ANU2
Feature Type	ORF, Verified
Description	Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress (1, 2)
Name Description	SECretory

Chromosomal Location	ChrIX:216658 to 217548 | ORF Map | GBrowse

	View Computational GO annotations for SEC28
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	ER to Golgi vesicle-mediated transport (IMP) late endosome to vacuole transport via multivesicular body sorting pathway (IMP, IPI) vesicle coating (IGI, IMP)
Cellular Component
Manually curated	COPI vesicle coat (IMP, ISS) colocalizes_with endosome (IDA)

Classical genetics
null	heat sensitivity: increased carboxypeptidase Y secretion: abnormal resistance to FK506: decreased resistance to staurosporine: decreased resistance to tunicamycin: decreased resistance to Calcofluor White: decreased resistance to mercaptoethanol: normal resistance to tunicamycin: normal resistance to L-1,4-dithiothreitol: decreased viable
Large-scale survey
null	proton accumulation: increased competitive fitness: decreased endocytosis: decreased haploinsufficient lifespan: increased replicative lifespan: increased resistance to tunicamycin: decreased resistance to propan-1-ol: decreased resistance to ethanol: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to pyocyanin: decreased resistance to quinine: decreased resistance to methyl methanesulfonate: decreased resistance to 1,4-dithiothreitol: decreased resistance to dimethyl sulfoxide: decreased resistance to (S)-lactic acid: decreased stress resistance: increased toxin resistance: decreased viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	612 total interaction(s) for 344 unique genes/features.
Physical Interactions	Affinity Capture-MS: 68 Affinity Capture-RNA: 2 Affinity Capture-Western: 5 Co-crystal Structure: 2 Protein-peptide: 1 Reconstituted Complex: 3 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 415 Phenotypic Suppression: 2 Positive Genetic: 87 Synthetic Growth Defect: 10 Synthetic Lethality: 12 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIX:216658 to 217548 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 2005-11-22
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..891 216658..217548 2011-02-03 2005-11-22
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001338