RPN2/YIL075C Summary Help

Standard Name RPN2 1
Systematic Name YIL075C
Alias SEN3 2
Feature Type ORF, Verified
Description Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress (2, 3, 4)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrIX:220700 to 217863 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RPN2 GO evidence and references
  View Computational GO annotations for RPN2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 9 genes
Resources
Classical genetics
conditional
Large-scale survey
null
reduction of function
Resources
238 total interaction(s) for 95 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 144
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 17
  • Biochemical Activity: 5
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 6
  • PCA: 1
  • Reconstituted Complex: 12
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 17
  • Phenotypic Enhancement: 2
  • Positive Genetic: 15
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 945
Molecular Weight (Da) 104,231
Isoelectric Point (pI) 6.07
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:220700 to 217863 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2838 220700..217863 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001337
References cited on this page View Complete Literature Guide for RPN2
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) DeMarini DJ, et al.  (1995) The yeast SEN3 gene encodes a regulatory subunit of the 26S proteasome complex required for ubiquitin-dependent protein degradation in vivo. Mol Cell Biol 15(11):6311-21
3) Kimura Y, et al.  (2003) N-Terminal modifications of the 19S regulatory particle subunits of the yeast proteasome. Arch Biochem Biophys 409(2):341-8
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76