NEO1/YIL048W Summary Help

Standard Name NEO1 1
Systematic Name YIL048W
Feature Type ORF, Verified
Description Putative aminophospholipid translocase (flippase); involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus (2, 3, 4 and see Summary Paragraph)
Name Description NEOmycin-resistance 1
Chromosomal Location
ChrIX:261437 to 264892 | ORF Map | GBrowse
Gene Ontology Annotations All NEO1 GO evidence and references
  View Computational GO annotations for NEO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
91 total interaction(s) for 79 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-Western: 2
  • PCA: 7
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 7
  • Negative Genetic: 49
  • Phenotypic Suppression: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 1,151
Molecular Weight (Da) 130,217
Isoelectric Point (pI) 6.43
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIX:261437 to 264892 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3456 261437..264892 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000001310

S. cerevisiae has five genes encoding type 4 P-type ATPases: NEO1, DRS2, DNF1, DNF2, and DNF3. The "P-type" designation indicates that these integral membrane proteins form a covalent aspartyl-phosphate catalytic intermediate during ATP hydrolysis (2 and references therein). Most P-type ATPases mediate the transport of small cations across biological membranes. However, members of the "type 4" subfamily are aminophospholipid translocases (flippases), rather than cation transporters, and move phospholipids from one side of a membrane bilayer to the other (reviewed in 5). Of the five S. cerevisiae type 4 P-type ATPases, only NEO1 is essential. Although the four other genes appear to have substantial functional overlap (any single DRS2/DNF gene confers cell viability) (2), they are distinct in their localization, specificity, and cofactor association.

Neo1p localizes primarily to endosomes and the Golgi apparatus, where it contributes to endocytosis and vacuolar biogenesis (3, 4). Because of the essential nature of this gene, Neo1p aminophospholipid translocase activity has not yet been experimentally demonstrated and substrate specificity is not currently known. Neo1p is associated with Mon2p (4), a Sec7p-family protein that shares very little sequence similarity with the non-catalytic subunits of other S. cerevisiae type 4 P-type ATPases.

The P-type ATPase superfamily is evolutionarily conserved, but the type 4 subfamily is found only in eukaryotes. Fourteen type 4 P-type ATPases have been characterized in humans (5 and references therein), including the DRS2 homolog, ATP8A1 (aka ATPase II) (6) and the DNF1/DNF2 homolog ATP8B1 (aka FIC1) (2). Mutations in ATP8B1 result in progressive familial intrahepatic cholestasis (Byler disease), benign recurrent intrahepatic cholestasis (BRIC), and intrahepatic cholestasis of pregnancy (ICP).

Last updated: 2007-03-01 Contact SGD

References cited on this page View Complete Literature Guide for NEO1
1) Prezant TR, et al.  (1996) Identification of an overexpressed yeast gene which prevents aminoglycoside toxicity. Microbiology 142 ( Pt 12)():3407-14
2) Hua Z, et al.  (2002) An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system. Mol Biol Cell 13(9):3162-77
3) Hua Z and Graham TR  (2003) Requirement for neo1p in retrograde transport from the Golgi complex to the endoplasmic reticulum. Mol Biol Cell 14(12):4971-83
4) Wicky S, et al.  (2004) Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system. Mol Cell Biol 24(17):7402-18
5) Paulusma CC and Oude Elferink RP  (2005) The type 4 subfamily of P-type ATPases, putative aminophospholipid translocases with a role in human disease. Biochim Biophys Acta 1741(1-2):11-24
6) Natarajan P, et al.  (2004) Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function. Proc Natl Acad Sci U S A 101(29):10614-9