NOT3/YIL038C Summary

NOT3 BASIC INFORMATION

Standard Name	NOT3
Systematic Name	YIL038C
Feature Type	ORF, Verified
Description	Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation (1, 2)

GO Annotations	All NOT3 GO evidence and references
	View Computational GO annotations for NOT3
Molecular Function
Manually curated	contributes_to ubiquitin-protein ligase activity (IMP)
Biological Process
Manually curated	nuclear-transcribed mRNA poly(A) tail shortening (IDA) protein ubiquitination (IMP) regulation of transcription from RNA polymerase II promoter (IPI)
Cellular Component
Manually curated	CCR4-NOT core complex (IPI) cytoplasm (IDA)

Regulatory Role
Regulatory modules	predicted: stressResponse ( 505 , 475 , 415 , 409 , 397 ) predicted: cellcycle ( 329 ) predicted: stressResponse ( 505 , 475 , 415 , 409 , 397 ) predicted: cellcycle ( 329 )

Mutant Phenotype	All NOT3 Phenotype details and references
Classical genetics
null	prion formation: increased resistance to hydroxyurea: decreased resistance to zymocin: decreased resistance to rapamycin: decreased viable
Large-scale survey
null	competitive fitness: decreased Can1p-GFP distribution: abnormal resistance to cisplatin: increased viable

Interactions	NOT3 All interactions details and references
	102 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Affinity Capture-Western: 14 Biochemical Activity: 3 Co-purification: 1 PCA: 11 Two-hybrid: 7
Genetic Interactions	Dosage Rescue: 3 Phenotypic Enhancement: 20 Phenotypic Suppression: 16 Synthetic Growth Defect: 5 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 4 Synthetic Rescue: 5

Sequence Information

ChrIX:282651 to 280141 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 1994-12-10 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2511	282651..280141	1994-12-10	1994-12-10

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001300

NOT3 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for NOT3

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NOT3]

1)	Liu HY, et al. (1998) The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively. EMBO J 17(4):1096-106
2)	Chen J, et al. (2001) Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J Mol Biol 314(4):683-94

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