FAA3/YIL009W Summary Help

Standard Name FAA3 1
Systematic Name YIL009W
Feature Type ORF, Verified
Description Long chain fatty acyl-CoA synthetase; activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery (2, 3 and see Summary Paragraph)
Name Description Fatty Acid Activation 1
Chromosomal Location
ChrIX:339344 to 341428 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All FAA3 GO evidence and references
  View Computational GO annotations for FAA3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 10 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
39 total interaction(s) for 33 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Co-purification: 1
  • PCA: 4

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 13
  • Phenotypic Enhancement: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 694
Molecular Weight (Da) 77,946
Isoelectric Point (pI) 9.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:339344 to 341428 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2085 339344..341428 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001271
SUMMARY PARAGRAPH for FAA3

In order for yeast to utilize fatty acids, either as an energy source (via beta-oxidation) or for essential processes such as phospholipid biosynthesis and protein myristoylation, the fatty acids must first be converted into activated intermediates, acyl-CoAs, through thioesterification of fatty acids with coenzyme A. When fatty acids are synthesized de novo, activation is part of the process of synthesis and is accomplished by the same fatty acid synthetase complex (Fas1p-Fas2p) that initiates and elongates the fatty acid chain. However, yeast cells can also utilize exogenous, imported fatty acids, an ability that becomes essential if the fatty acid synthetase complex is inactivated by mutation or specific inhibitors such as cerulenin. These exogenous fatty acids are activated by one of five characterized yeast acyl-CoA synthetases: Faa1p, Faa2p, Faa3p, Faa4p, or Fat1p (see 4 and 5 for review).

Purified Faa3p has been demonstrated to have acyl-CoA synthetase activity. The in vitro activity of Faa3p is significantly lower than that of Faa1p or Faa2p for medium or long chain fatty acids (C9:0-C18:0)(2). Unlike Faa1p or Faa2p, Faa3p demonstrates in vitro activity on very long chain fatty acids such as C22:0 and lignoceric acid (C24:0) (2); however, mutant analysis indicates that, under most conditions, Fat1p is the major acyl-CoA synthetase for very long chain fatty acids (6, 7).

Last updated: 2010-02-11 Contact SGD

References cited on this page View Complete Literature Guide for FAA3
1) Johnson DR, et al.  (1994) Saccharomyces cerevisiae contains four fatty acid activation (FAA) genes: an assessment of their role in regulating protein N-myristoylation and cellular lipid metabolism. J Cell Biol 127(3):751-62
2) Knoll LJ, et al.  (1994) Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p. J Biol Chem 269(23):16348-56
3) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4) Hettema EH and Tabak HF  (2000) Transport of fatty acids and metabolites across the peroxisomal membrane. Biochim Biophys Acta 1486(1):18-27
5) Black PN and Dirusso CC  (2007) Yeast acyl-CoA synthetases at the crossroads of fatty acid metabolism and regulation. Biochim Biophys Acta 1771(3):286-98
6) Choi JY and Martin CE  (1999) The Saccharomyces cerevisiae FAT1 gene encodes an acyl-CoA synthetase that is required for maintenance of very long chain fatty acid levels. J Biol Chem 274(8):4671-83
7) Watkins PA, et al.  (1998) Disruption of the Saccharomyces cerevisiae FAT1 gene decreases very long-chain fatty acyl-CoA synthetase activity and elevates intracellular very long-chain fatty acid concentrations. J Biol Chem 273(29):18210-9