BET1/YIL004C Summary Help

Standard Name BET1 1
Systematic Name YIL004C
Alias SLY12
Feature Type ORF, Verified
Description Type II membrane protein required for vesicular transport; required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins (2 and see Summary Paragraph)
Name Description Blocked Early in Transport 1
Chromosomal Location
ChrIX:348505 to 347946 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BET1 GO evidence and references
  View Computational GO annotations for BET1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
113 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 34
  • Biochemical Activity: 1
  • Co-fractionation: 2
  • Co-purification: 2
  • FRET: 2
  • PCA: 2
  • Reconstituted Complex: 25
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 24
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 4
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 142
Molecular Weight (Da) 15,720
Isoelectric Point (pI) 9.17
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIX:348505 to 347946 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..11 348505..348495 2011-02-03 1994-12-10
Intron 12..142 348494..348364 2011-02-03 1994-12-10
CDS 143..560 348363..347946 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001266

BET1 encodes a v-SNAP receptor (a.k.a. v-SNARE, a soluble NSF[N-ethylmaleimide-sensitive fusion protein] attachment protein receptor) involved in transport between the endoplasmic reticulum and the Golgi (1). In vitro experiments show that Bet1p is involved in both forward and retrograde transport (3). If vesicular transport is conceptually divided into three steps, vesicle budding, vesicle docking, and membrane fusion, Bet1p is likely involved in the membrane fusion step (4). Bet1p is a cytoplasmically-oriented membrane protein that co-fractionates with v-SNAP receptor Bos1p (a suppressor of bet1 mutants) and the endoplasmic reticulum membrane; a tagged version of the protein is found in the early Golgi (5, 6). Overexpression of Bet1p compensates for defects in Sec22p and the ras-like G-protein Ypt1p (5, 7, 2). Bet1p has some structural similarity to synaptobrevins in other eukaryotes, and a domain with potential to be involved in coiled-coil interactions is homologous to a region of the neuronal protein SNAP-25 (8, 9).

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for BET1
1) Newman AP and Ferro-Novick S  (1987) Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection. J Cell Biol 105(4):1587-94
2) Newman AP, et al.  (1990) BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex. Mol Cell Biol 10(7):3405-14
3) Spang A and Schekman R  (1998) Reconstitution of retrograde transport from the Golgi to the ER in vitro. J Cell Biol 143(3):589-99
4) Cao X, et al.  (1998) Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins. EMBO J 17(8):2156-65
5) Newman AP, et al.  (1992) Bos1p, a membrane protein required for ER to Golgi transport in yeast, co-purifies with the carrier vesicles and with Bet1p and the ER membrane. EMBO J 11(10):3609-17
6) Ossipov D, et al.  (1999) Yeast ER-Golgi v-SNAREs Bos1p and Bet1p differ in steady-state localization and targeting. J Cell Sci 112 ( Pt 22):4135-42
7) Ossig R, et al.  (1991) The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport. Mol Cell Biol 11(6):2980-93
8) Dascher C, et al.  (1991) Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily. Mol Cell Biol 11(2):872-85
9) Stone S, et al.  (1997) Bet1p activates the v-SNARE Bos1p. Mol Biol Cell 8(7):1175-81