IMD2/YHR216W Summary

IMD2/YHR216W Summary

Standard Name	IMD2 1
Systematic Name	YHR216W
Alias	PUR5 2
Feature Type	ORF, Verified
Description	Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in GTP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expression is repressed by nutrient limitation; IMD2 has a paralog, YAR073W/YAR075W, that arose from a segmental duplication (1, 3, 4, 5, 6)
Name Description	IMP Dehydrogenase 1

Chromosomal Location	ChrVIII:554396 to 555967 \| ORF Map \| GBrowse

Gene Ontology Annotations All IMD2 GO evidence and references

	View Computational GO annotations for IMD2
Molecular Function
Manually curated	chromatin binding (IDA) IMP dehydrogenase activity (TAS)
Biological Process
Manually curated	GTP biosynthetic process (TAS)
Cellular Component
Manually curated	chromatin (IDA)
High-throughput	cytoplasm (IDA)

Pathways	de novo biosynthesis of purine nucleotides superpathway of histidine, purine, and pyrimidine biosynthesis superpathway of purine biosynthesis and salvage pathways

Mutant phenotypes All IMD2 Phenotype evidence and references

Classical genetics
null	resistance to mycophenolic acid: decreased viable
overexpression	resistance to Mycophenolic acid: increased
unspecified	resistance to mycophenolic acid: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All IMD2 Interaction evidence and references

	71 total interaction(s) for 61 unique genes/features.
Physical Interactions	Affinity Capture-MS: 39 Affinity Capture-RNA: 2 Biochemical Activity: 2 Co-fractionation: 1 Co-purification: 1 Protein-peptide: 1 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 5 Negative Genetic: 9 Positive Genetic: 8 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All IMD2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVIII:554396 to 555967 | |

SGD ORF map

Last Update

Coordinates: 2005-11-07 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1572	554396..555967	2005-11-07	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001259

References cited on this page View Complete Literature Guide for IMD2

1)	Escobar-Henriques M and Daignan-Fornier B (2001) Transcriptional regulation of the yeast gmp synthesis pathway by its end products. J Biol Chem 276(2):1523-30
2)	Shaw RJ and Reines D (2000) Saccharomyces cerevisiae transcription elongation mutants are defective in PUR5 induction in response to nucleotide depletion. Mol Cell Biol 20(20):7427-37
3)	Shaw RJ, et al. (2001) Regulation of an IMP dehydrogenase gene and its overexpression in drug-sensitive transcription elongation mutants of yeast. J Biol Chem 276(35):32905-16
4)	Hyle JW, et al. (2003) Functional distinctions between IMP dehydrogenase genes in providing mycophenolate resistance and guanine prototrophy to yeast. J Biol Chem 278(31):28470-8
5)	Escobar-Henriques M, et al. (2003) Transcription initiation of the yeast IMD2 gene is abolished in response to nutrient limitation through a sequence in its coding region. Mol Cell Biol 23(17):6279-90
6)	Katju V, et al. (2009) Variation in gene duplicates with low synonymous divergence in Saccharomyces cerevisiae relative to Caenorhabditis elegans. Genome Biol 10(7):R75