MNL1/YHR204W Summary Help

Standard Name MNL1 1
Systematic Name YHR204W
Alias HTM1 2
Feature Type ORF, Verified
Description Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation (1, 2, 3, 4)
Name Description MaNnosidase-Like protein 1
Chromosomal Location
ChrVIII:506319 to 508709 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MNL1 GO evidence and references
  View Computational GO annotations for MNL1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
32 total interaction(s) for 20 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 7
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 9
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 796
Molecular Weight (Da) 91,245
Isoelectric Point (pI) 4.82
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:506319 to 508709 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2391 506319..508709 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001247
References cited on this page View Complete Literature Guide for MNL1
1) Nakatsukasa K, et al.  (2001) Mnl1p, an alpha -mannosidase-like protein in yeast Saccharomyces cerevisiae, is required for endoplasmic reticulum-associated degradation of glycoproteins. J Biol Chem 276(12):8635-8
2) Jakob CA, et al.  (2001) Htm1p, a mannosidase-like protein, is involved in glycoprotein degradation in yeast. EMBO Rep 2(5):423-30
3) Clerc S, et al.  (2009) Htm1 protein generates the N-glycan signal for glycoprotein degradation in the endoplasmic reticulum. J Cell Biol 184(1):159-72
4) Gauss R, et al.  (2011) A complex of pdi1p and the mannosidase htm1p initiates clearance of unfolded glycoproteins from the endoplasmic reticulum. Mol Cell 42(6):782-93