RPN10/YHR200W Summary Help

RPN10 BASIC INFORMATION

Standard Name RPN10 1
Systematic Name YHR200W
Alias MCB1 2 , SUN1 3
Feature Type ORF, Verified
Description Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein (1, 4, 5, 6)
Name Description Regulatory Particle Non-ATPase 1
GO Annotations All RPN10 GO evidence and references
    View Computational GO annotations for RPN10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All RPN10 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions RPN10 All interactions details and references
973 total interaction(s) for 565 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 513
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 21
  • Biochemical Activity: 2
  • Co-fractionation: 5
  • Co-purification: 10
  • Far Western: 2
  • Reconstituted Complex: 8
  • Two-hybrid: 2
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 157
  • Phenotypic Suppression: 50
  • Synthetic Growth Defect: 153
  • Synthetic Lethality: 44
  • Synthetic Rescue: 2
Sequence Information
ChrVIII:499079 to 499885 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..807 499079..499885 2005-11-07 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001243

RPN10 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
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  • Functional Analysis

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Expression Summary histogram

ADDITIONAL INFORMATION for RPN10

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RPN10]

1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) van Nocker S, et al.  (1996) The multiubiquitin-chain-binding protein Mcb1 is a component of the 26S proteasome in Saccharomyces cerevisiae and plays a nonessential, substrate-specific role in protein turnover. Mol Cell Biol 16(11):6020-8
3) Kominami K, et al.  (1997) Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1. Mol Biol Cell 8(1):171-87
4) Glickman MH, et al.  (1998) A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3. Cell 94(5):615-23
5) Elsasser S, et al.  (2002) Proteasome subunit Rpn1 binds ubiquitin-like protein domains. Nat Cell Biol 4(9):725-30
6) Deveraux Q, et al.  (1994) A 26 S protease subunit that binds ubiquitin conjugates. J Biol Chem 269(10):7059-61