RPN10/YHR200W Summary Help

Standard Name RPN10 1
Systematic Name YHR200W
Alias MCB1 2 , SUN1 3
Feature Type ORF, Verified
Description Non-ATPase base subunit of the 19S RP of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the regulatory particle (RP); binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein (1, 4, 5, 6)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrVIII:499079 to 499885 | ORF Map | GBrowse
Gene Ontology Annotations All RPN10 GO evidence and references
  View Computational GO annotations for RPN10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Classical genetics
Large-scale survey
1228 total interaction(s) for 656 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 525
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 41
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-fractionation: 5
  • Co-purification: 12
  • Far Western: 2
  • Reconstituted Complex: 15
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 2
  • Negative Genetic: 309
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 4
  • Positive Genetic: 77
  • Synthetic Growth Defect: 126
  • Synthetic Lethality: 49
  • Synthetic Rescue: 43

Expression Summary
Length (a.a.) 268
Molecular Weight (Da) 29,747
Isoelectric Point (pI) 4.56
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:499079 to 499885 | ORF Map | GBrowse
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..807 499079..499885 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001243
References cited on this page View Complete Literature Guide for RPN10
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) van Nocker S, et al.  (1996) The multiubiquitin-chain-binding protein Mcb1 is a component of the 26S proteasome in Saccharomyces cerevisiae and plays a nonessential, substrate-specific role in protein turnover. Mol Cell Biol 16(11):6020-8
3) Kominami K, et al.  (1997) Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1. Mol Biol Cell 8(1):171-87
4) Glickman MH, et al.  (1998) A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3. Cell 94(5):615-23
5) Elsasser S, et al.  (2002) Proteasome subunit Rpn1 binds ubiquitin-like protein domains. Nat Cell Biol 4(9):725-30
6) Deveraux Q, et al.  (1994) A 26 S protease subunit that binds ubiquitin conjugates. J Biol Chem 269(10):7059-61