KOG1/YHR186C Summary Help

Standard Name KOG1 1
Systematic Name YHR186C
Alias LAS24 2
Feature Type ORF, Verified
Description Subunit of TORC1; TORC1 is a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors (1, 3, 4, 5 and see Summary Paragraph)
Name Description Kontroller Of Growth 1
Chromosomal Location
ChrVIII:480672 to 475999 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All KOG1 GO evidence and references
  View Computational GO annotations for KOG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Classical genetics
conditional
null
unspecified
Large-scale survey
conditional
null
reduction of function
Resources
116 total interaction(s) for 77 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 42
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 33
  • Co-fractionation: 1
  • Co-purification: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 22

Genetic Interactions
  • Dosage Rescue: 7
  • Negative Genetic: 3
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,557
Molecular Weight (Da) 177,608
Isoelectric Point (pI) 7.78
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:480672 to 475999 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4674 480672..475999 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001229
SUMMARY PARAGRAPH for KOG1

KOG1 encodes an essential protein that is a component of the TOR complex 1 (TORC1; 1). TORC1 mediates cell growth in response to nutrient availability and cellular stresses by regulating protein synthesis, ribosome biogenesis, autophagy, transcriptional activation, meiosis, cell cycling, nutrient permease sorting and turnover (reviewed in 6, 7). In addition to Kog1p, TORC1 consists of Lst8p, Tco89p and either Tor1p or Tor2p (1, 5). Although TORC1 can contain either Tor1p or Tor2p, Kog1p interacts preferentially with Tor1p (4). TORC1 is sensitive to the drug rapamycin, which forms a complex with Fpr1p that binds to the Tor protein and inhibits complex activity (8, 1). Kog1p depletion mimics rapamycin treatment and cells display the starvation-like phenotypes of cell growth arrest, altered cell morphology, reduction in protein synthesis, glycogen accumulation, and upregulation in the transcription of nitrogen catabolite repressed and retrograde response genes (1).

Kog1p has a molecular weight of 176 kDa and contains four internal HEAT repeats and seven C-terminal WD-40 repeats (1). KOG1 is conserved from yeast to man and is the homolog of the mammalian TOR regulatory protein RAPTOR/mKOG1 (1, 9). TORC1 is also structurally and functionally conserved in higher eukaryotes and has been named the 'nutrient-sensitive complex' in mammals (1, 9). In Drosophila, C. elegans, and mammals, TORC1 activity has been shown to participate in the additional processes of apoptosis, hypoxia, and aging (reviewed in 10, 6).

Last updated: 2005-11-03 Contact SGD

References cited on this page View Complete Literature Guide for KOG1
1) Loewith R, et al.  (2002) Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell 10(3):457-68
2) Araki T, et al.  (2005) LAS24/KOG1, a component of the TOR complex 1 (TORC1), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast. Genes Genet Syst 80(5):325-43
3) Jacinto E and Hall MN  (2003) Tor signalling in bugs, brain and brawn. Nat Rev Mol Cell Biol 4(2):117-26
4) Wedaman KP, et al.  (2003) Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae. Mol Biol Cell 14(3):1204-20
5) Reinke A, et al.  (2004) TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem 279(15):14752-62
6) Martin DE and Hall MN  (2005) The expanding TOR signaling network. Curr Opin Cell Biol 17(2):158-66
7) Inoki K, et al.  (2005) Signaling by target of rapamycin proteins in cell growth control. Microbiol Mol Biol Rev 69(1):79-100
8) Stan R, et al.  (1994) Interaction between FKBP12-rapamycin and TOR involves a conserved serine residue. J Biol Chem 269(51):32027-30
9) Kim DH, et al.  (2002) mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Cell 110(2):163-75
10) Bjornsti MA and Houghton PJ  (2004) The TOR pathway: a target for cancer therapy. Nat Rev Cancer 4(5):335-48