STB5/YHR178W Summary

STB5/YHR178W Summary

Standard Name	STB5
Systematic Name	YHR178W
Feature Type	ORF, Verified
Description	Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro (1, 2, 3)
Name Description	Sin Three Binding protein

Chromosomal Location	ChrVIII:459299 to 461530 \| ORF Map \| GBrowse

Gene Ontology Annotations All STB5 GO evidence and references

	View Computational GO annotations for STB5
Molecular Function
Manually curated	sequence-specific DNA binding transcription factor activity (IDA, IGI, IMP, IPI, ISS)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	regulation of transcription from RNA polymerase II promoter in response to oxidative stress (IMP) response to xenobiotic stimulus (IMP) transcription, DNA-dependent (IDA)
Cellular Component
Manually curated	nucleus (IPI)

Regulatory Role

Binding motifs	Predicted STB5 Binding Site Locations
	CGG 4 [Search intergenic regions for CGG] CGGNSTTATA 5 [Search intergenic regions for CGGNSTTATA] CGGNVHTA 6 [Search intergenic regions for CGGNVHTA]

Resources	ScerTF \| YEASTRACT \| YeTFaSCo

Mutant phenotypes All STB5 Phenotype evidence and references

Classical genetics
null	cold sensitivity: increased oxidative stress resistance: decreased resistance to caffeine: decreased resistance to diamide: decreased resistance to capric acid: decreased resistance to sirolimus: increased utilization of nitrogen source: decreased vegetative growth: decreased viable
overexpression	NADPH accumulation: increased resistance to diamide: increased
Large-scale survey
null	auxotrophy competitive fitness: decreased resistance to oligomycin: normal resistance to 4-nitroquinoline N-oxide: normal resistance to caffeine: decreased resistance to arsenite(3-): decreased resistance to 5-fluorouracil: decreased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to cycloheximide: decreased resistance to rhodamine 6-G: normal resistance to chloramphenicol: normal resistance to ketoconazole: normal resistance to acetaldehyde: decreased resistance to hygromycin B: decreased resistance to acrolein: decreased resistance to sorbate: decreased resistance to amitrole: decreased starvation resistance: decreased transposable element transposition: increased utilization of nitrogen source: absent vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All STB5 Interaction evidence and references

	257 total interaction(s) for 224 unique genes/features.
Physical Interactions	Affinity Capture-Western: 2 Reconstituted Complex: 1 Two-hybrid: 3
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 174 Positive Genetic: 45 Synthetic Growth Defect: 19 Synthetic Lethality: 11
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All STB5 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVIII:459299 to 461530 | |

SGD ORF map

Last Update

Coordinates: 2005-11-07 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2232	459299..461530	2005-11-07	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001221

References cited on this page View Complete Literature Guide for STB5

1)	Kasten MM and Stillman DJ (1997) Identification of the Saccharomyces cerevisiae genes STB1-STB5 encoding Sin3p binding proteins. Mol Gen Genet 256(4):376-86
2)	Akache B, et al. (2004) Complex interplay among regulators of drug resistance genes in Saccharomyces cerevisiae. J Biol Chem 279(27):27855-60
3)	Larochelle M, et al. (2006) Oxidative stress-activated zinc cluster protein Stb5 has dual activator/repressor functions required for pentose phosphate pathway regulation and NADPH production. Mol Cell Biol 26(17):6690-701
4)	Akache B and Turcotte B (2002) New regulators of drug sensitivity in the family of yeast zinc cluster proteins. J Biol Chem 277(24):21254-60
5)	Harbison CT, et al. (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
6)	Badis G, et al. (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87