ENO2/YHR174W Summary Help

Standard Name ENO2 1
Systematic Name YHR174W
Feature Type ORF, Verified
Description Enolase II, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression induced in response to glucose; ENO2 has a paralog, ENO1, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description ENO2 1
Gene Product Alias enolase 1
Chromosomal Location
ChrVIII:451327 to 452640 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ENO2 GO evidence and references
  View Computational GO annotations for ENO2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 15 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
overexpression
Resources
183 total interaction(s) for 164 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 84
  • Affinity Capture-RNA: 5
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 6
  • Protein-peptide: 8
  • Reconstituted Complex: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 57
  • Positive Genetic: 9
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 437
Molecular Weight (Da) 46,914
Isoelectric Point (pI) 5.88
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:451327 to 452640 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1314 451327..452640 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001217
SUMMARY PARAGRAPH for ENO2

ENO1 and ENO2 are the two S. cerevisiae genes encoding phosphopyruvate hydratase, which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis. The enolase enzymes function as dimeric phosphopyruvate hydratase complexes (1). Replacement of His373 with asparagine (H373N enolase) or phenylalanine (H373F enolase) reduces enzymatic activity of Eno1p to ca. 10% and 0.0003% of its native enzyme activity, respectively (4).

Log phase cells grown on glucose contain 20-fold more Eno2p than Eno1p, whereas cells grown on ethanol or glycerol plus lactate contain similar amounts of both proteins (1). Enolase catalyses the first common step of glycolysis and gluconeogenesis. During gluconeogenesis, ENO1 and ENO2 catalyze the reverse reaction --- the synthesis of phosphoenolpyruvate from 2-phosphoglycerate (5, ,6, 7). This reaction is important for growth on non-sugar carbon sources like ethanol, glycerol, or peptone, when the gluconeogenesis pathway is used to synthesize glucose.

The reactions of gluconeogenesis, shown here, mediate conversion of pyruvate to glucose, which is the opposite of glycolysis, the formation of pyruvate from glucose. While these two pathways have several reactions in common, they are not the exact reverse of each other. As the glycolytic enzymes phosphofructokinase (Pfk1p, Pfk2p) and pyruvate kinase (Cdc19p) only function in the forward direction, the gluconeogenesis pathway replaces those steps with the enzymes pyruvate carboxylase (Pyc1p, Pyc2p) and phosphoenolpyruvate carboxykinase (Pck1p) -generating oxaloacetate as an intermediate from pyruvate to phosphoenolpyruvate- and also the enzyme fructose-1,6-bisphosphatase (Fbp1p) (reviewed in 8). Overall, the gluconeogenic reactions convert two molecules of pyruvate to a molecule of glucose, with the expenditure of six high-energy phosphate bonds, four from ATP and two from GTP.

Last updated: 2005-07-05 Contact SGD

References cited on this page View Complete Literature Guide for ENO2
1) McAlister L and Holland MJ  (1982) Targeted deletion of a yeast enolase structural gene. Identification and isolation of yeast enolase isozymes. J Biol Chem 257(12):7181-8
2) Entian KD, et al.  (1987) Studies on the regulation of enolases and compartmentation of cytosolic enzymes in Saccharomyces cerevisiae. Biochim Biophys Acta 923(2):214-21
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Brewer JM, et al.  (1997) Effect of site-directed mutagenesis of His373 of yeast enolase on some of its physical and enzymatic properties. Biochim Biophys Acta 1340(1):88-96
5) Cohen R, et al.  (1987) Transcription of the constitutively expressed yeast enolase gene ENO1 is mediated by positive and negative cis-acting regulatory sequences. Mol Cell Biol 7(8):2753-61
6) Wold F and Ballou CE  (1957) Studies on the enzyme enolase. I. Equilibrium studies. J Biol Chem 227(1):301-12
7) Wold F and Ballou CE  (1957) Studies on the enzyme enolase. II. Kinetic studies. J Biol Chem 227(1):313-28
8) Klein CJ, et al.  (1998) Glucose control in Saccharomyces cerevisiae: the role of Mig1 in metabolic functions. Microbiology 144 ( Pt 1)():13-24