CDC23/YHR166C Summary Help

Standard Name CDC23 1
Systematic Name YHR166C
Feature Type ORF, Verified
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition (2 and see Summary Paragraph)
Name Description Cell Division Cycle 3
Chromosomal Location
ChrVIII:439050 to 437170 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CDC23 GO evidence and references
  View Computational GO annotations for CDC23
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
210 total interaction(s) for 104 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 56
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 28
  • Biochemical Activity: 2
  • Co-localization: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 4
  • Negative Genetic: 57
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 22
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 626
Molecular Weight (Da) 73,113
Isoelectric Point (pI) 5.18
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:439050 to 437170 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1881 439050..437170 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001209
SUMMARY PARAGRAPH for CDC23

CDC23 (APC8) is an essential member of the anaphase-promoting complex (APC), an E3 ubiquitin ligase in the ubiquitin-mediated proteolysis pathway (2). This protein contains tetratrico peptide repeats (TPR), a protein-protein interaction motif (4). The APC ubiquitin ligase helps regulate the metaphase/anaphase transition and exit from mitosis/G1 entry through ubiquitination of various substrates. These include mitotic cyclins, the sister chromatid separation inhibitor Pds1p, the Kip1p and Cin8p motor proteins, Cdc5p, and the spindle disassembly factor, Ase1p (5, 6, 7, 8, 9, 10). Cdc23p has been shown to interact with other APC subunits including Mnd2p, Cdc16p and Cdc27p, as well as the N-terminus of Clb2p (11, 12, 13). Temperature-sensitive mutants of CDC23 arrest as large-budded cells with the nucleus at the neck and are defective in ubiquitination of Clb2p at non-permissive temperature (10,14,15,16). Cdc23p localizes to the nucleus, kinetochores/microtubule ends, and mitotic spindle in budding yeast (17).

Many thanks to Patricia Melloy for writing this gene summary.

Last updated: 2004-11-18 Contact SGD

References cited on this page View Complete Literature Guide for CDC23
1) Hartwell LH, et al.  (1973) Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants. Genetics 74(2):267-286
2) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
3) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
4) Lamb JR, et al.  (1995) Tetratrico peptide repeat interactions: to TPR or not to TPR? Trends Biochem Sci 20(7):257-9
5) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
6) Cohen-Fix O, et al.  (1996) Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev 10(24):3081-93
7) Shirayama M, et al.  (1998) The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J 17(5):1336-49
8) Gordon DM and Roof DM  (2001) Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc Natl Acad Sci U S A 98(22):12515-20
9) Hildebrandt ER and Hoyt MA  (2001) Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. Mol Biol Cell 12(11):3402-16
10) Zachariae W and Nasmyth K  (1996) TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast. Mol Biol Cell 7(5):791-801
11) Lamb JR, et al.  (1994) Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis. EMBO J 13(18):4321-8
12) Hall MC, et al.  (2003) Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex. J Biol Chem 278(19):16698-705
13) Meyn MA 3rd, et al.  (2002) The destruction box of the cyclin Clb2 binds the anaphase-promoting complex/cyclosome subunit Cdc23. Arch Biochem Biophys 407(2):189-95
14) Sikorski RS, et al.  (1991) TPR proteins as essential components of the yeast cell cycle. Cold Spring Harb Symp Quant Biol 56():663-73
15) Sikorski RS, et al.  (1993) p62cdc23 of Saccharomyces cerevisiae: a nuclear tetratricopeptide repeat protein with two mutable domains. Mol Cell Biol 13(2):1212-21
16) Irniger S, et al.  (1995) Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast. Cell 81(2):269-78
17) Melloy PG and Holloway SL  (2004) Changes in the localization of the Saccharomyces cerevisiae anaphase-promoting complex upon microtubule depolymerization and spindle checkpoint activation. Genetics 167(3):1079-94