CDC23/YHR166C Summary Help

CDC23 BASIC INFORMATION

Standard Name CDC23
Systematic Name YHR166C
Feature Type ORF, Verified
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition (1 and see Summary Paragraph)
Name Description Cell Division Cycle 2
GO Annotations All CDC23 GO evidence and references
    View Computational GO annotations for CDC23
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All CDC23 Phenotype details and references
Classical genetics
conditional
Large-scale survey
null
repressible
Interactions CDC23 All interactions details and references
135 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 54
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 24
  • Biochemical Activity: 2
  • Co-localization: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 17
Genetic Interactions
  • Dosage Rescue: 3
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 18
  • Synthetic Rescue: 2
Sequence Information
ChrVIII:439052 to 437172 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1881 439052..437172 2005-11-07 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001209

CDC23 RESOURCES

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SGD ORF map
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Expression Summary histogram

ADDITIONAL INFORMATION for CDC23

SUMMARY PARAGRAPH for CDC23

CDC23 (APC8) is an essential member of the anaphase-promoting complex (APC), an E3 ubiquitin ligase in the ubiquitin-mediated proteolysis pathway (1). This protein contains tetratrico peptide repeats (TPR), a protein-protein interaction motif (3). The APC ubiquitin ligase helps regulate the metaphase/anaphase transition and exit from mitosis/G1 entry through ubiquitination of various substrates. These include mitotic cyclins, the sister chromatid separation inhibitor Pds1p, the Kip1p and Cin8p motor proteins, Cdc5p, and the spindle disassembly factor, Ase1p (4, 5, 6, 7, 8, 9). Cdc23p has been shown to interact with other APC subunits including Mnd2p, Cdc16p and Cdc27p, as well as the N-terminus of Clb2p (10, 11, 12). Temperature-sensitive mutants of CDC23 arrest as large-budded cells with the nucleus at the neck and are defective in ubiquitination of Clb2p at non-permissive temperature (9,13,14,15). Cdc23p localizes to the nucleus, kinetochores/microtubule ends, and mitotic spindle in budding yeast (16).

Many thanks to Patricia Melloy for writing this gene summary.

Last updated: 2004-11-18

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CDC23]

1) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
2) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
3) Lamb JR, et al.  (1995) Tetratrico peptide repeat interactions: to TPR or not to TPR? Trends Biochem Sci 20(7):257-9
4) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
5) Cohen-Fix O, et al.  (1996) Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev 10(24):3081-93
6) Shirayama M, et al.  (1998) The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J 17(5):1336-49
7) Gordon DM and Roof DM  (2001) Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc Natl Acad Sci U S A 98(22):12515-20
8) Hildebrandt ER and Hoyt MA  (2001) Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. Mol Biol Cell 12(11):3402-16
9) Zachariae W and Nasmyth K  (1996) TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast. Mol Biol Cell 7(5):791-801
10) Lamb JR, et al.  (1994) Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis. EMBO J 13(18):4321-8
11) Hall MC, et al.  (2003) Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex. J Biol Chem 278(19):16698-705
12) Meyn MA 3rd, et al.  (2002) The destruction box of the cyclin Clb2 binds the anaphase-promoting complex/cyclosome subunit Cdc23. Arch Biochem Biophys 407(2):189-95
13) Sikorski RS, et al.  (1991) TPR proteins as essential components of the yeast cell cycle. Cold Spring Harb Symp Quant Biol 56:663-73
14) Sikorski RS, et al.  (1993) p62cdc23 of Saccharomyces cerevisiae: a nuclear tetratricopeptide repeat protein with two mutable domains. Mol Cell Biol 13(2):1212-21
15) Irniger S, et al.  (1995) Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast. Cell 81(2):269-78
16) Melloy PG and Holloway SL  (2004) Changes in the localization of the Saccharomyces cerevisiae anaphase-promoting complex upon microtubule depolymerization and spindle checkpoint activation. Genetics 167(3):1079-94