Standard Name	PRP8
Systematic Name	YHR165C
Alias	DBF3 , DNA39 1 , RNA8 , SLT21 2 , USA2 3
Feature Type	ORF, Verified
Description	Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing; mutations of human Prp8 cause retinitis pigmentosa (4, 5, 6)
Name Description	Pre-mRNA Processing 7

Chromosomal Location	ChrVIII:436948 to 429707 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for PRP8
Molecular Function
Manually curated	contributes_to second spliceosomal transesterification activity (IMP) U5 snRNA binding (IDA)
Biological Process
Manually curated	assembly of spliceosomal tri-snRNP (IMP) generation of catalytic spliceosome for second transesterification step (IMP) mRNA 3'-splice site recognition (IMP)
Cellular Component
Manually curated	nucleus (IDA) U4/U6 x U5 tri-snRNP complex (IDA) U5 snRNP (IDA)
High-throughput	nucleus (IDA)

Classical genetics
conditional	RPS14A pre-mRNA accumulation: increased ACT1 pre-mRNA accumulation: increased heat sensitivity: increased sporulation: absent
null	inviable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	316 total interaction(s) for 117 unique genes/features.
Physical Interactions	Affinity Capture-MS: 216 Affinity Capture-RNA: 2 Affinity Capture-Western: 21 Co-crystal Structure: 1 Co-fractionation: 6 Co-localization: 2 Co-purification: 2 Protein-peptide: 2 Reconstituted Complex: 2 Two-hybrid: 25
Genetic Interactions	Dosage Lethality: 2 Dosage Rescue: 2 Negative Genetic: 7 Phenotypic Enhancement: 1 Synthetic Growth Defect: 5 Synthetic Lethality: 11 Synthetic Rescue: 9
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVIII:436948 to 429707 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..7242 436948..429707 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001208