DNA2/YHR164C Summary 
| Standard Name | DNA2 1 |
|---|---|
| Systematic Name | YHR164C |
| Alias | WEB2 |
| Feature Type | ORF, Verified |
| Description | Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress (2, 3, 4, 5, 6, 7, 8, 9) |
| Name Description | DNA synthesis defective 1 |
| Chromosomal Location | |
|---|---|
| Note: this feature is encoded on the Crick strand. | |
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Gene Ontology Annotations All DNA2 GO evidence and references
| View Computational GO annotations for DNA2 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated | |
| High-throughput |
Mutant phenotypes All DNA2 Phenotype evidence and references
| Classical genetics | |
|---|---|
| conditional | |
| overexpression | |
| reduction of function | |
| repressible | |
| unspecified | |
| Large-scale survey | |
| conditional | |
| null | |
| overexpression | |
| reduction of function | |
| repressible | |
| Resources |
interactions All DNA2 Interaction evidence and references
| 135 total interaction(s) for 76 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
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Expression Summary
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| Resources |
Protein Information All DNA2 Protein evidence and references
| Localization | |
|---|---|
| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
sequence information
| Note: this feature is encoded on the Crick strand. | |||||||||||||
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| Retrieve sequences | |||||||||||||
Analyze Sequence
| S288C only | |
|---|---|
| S288C vs. other species | |
| S288C vs. other strains |
Resources
| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000001207 |
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References cited on this page View Complete Literature Guide for DNA2
| 1) | Dumas LB, et al. (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6 |
| 2) | Budd ME and Campbell JL (2000) The pattern of sensitivity of yeast dna2 mutants to DNA damaging agents suggests a role in DSB and postreplication repair pathways. Mutat Res 459(3):173-86 |
| 3) | Budd ME and Campbell JL (1997) A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Mol Cell Biol 17(4):2136-42 |
| 4) | Schlesinger MB and Formosa T (2000) POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae. Genetics 155(4):1593-606 |
| 5) | Bae SH, et al. (1998) Dna2 of Saccharomyces cerevisiae possesses a single-stranded DNA-specific endonuclease activity that is able to act on double-stranded DNA in the presence of ATP. J Biol Chem 273(41):26880-90 |
| 6) | Bae SH and Seo YS (2000) Characterization of the enzymatic properties of the yeast dna2 Helicase/endonuclease suggests a new model for Okazaki fragment processing. J Biol Chem 275(48):38022-31 |
| 7) | Kosugi S, et al. (2009) Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs. Proc Natl Acad Sci U S A 106(25):10171-6 |
| 8) | Balakrishnan L, et al. (2010) Dna2 exhibits a unique strand end-dependent helicase function. J Biol Chem 285(50):38861-8 |
| 9) | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
