SPO12/YHR152W Summary Help

Standard Name SPO12 1
Systematic Name YHR152W
Alias SDB21 2
Feature Type ORF, Verified
Description Nucleolar protein of unknown function; positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis; SPO12 has a paralog, BNS1, that arose from the whole genome duplication (3, 4, 5, 6)
Name Description SPOrulation 7
Chromosomal Location
ChrVIII:401434 to 401955 | ORF Map | GBrowse
Genetic position: 83 cM
Gene Ontology Annotations All SPO12 GO evidence and references
  View Computational GO annotations for SPO12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 12 genes
Classical genetics
reduction of function
Large-scale survey
168 total interaction(s) for 121 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 27

Genetic Interactions
  • Dosage Rescue: 21
  • Negative Genetic: 37
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 10
  • Positive Genetic: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 14
  • Synthetic Rescue: 7

Expression Summary
Length (a.a.) 173
Molecular Weight (Da) 19,912
Isoelectric Point (pI) 10.19
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:401434 to 401955 | ORF Map | GBrowse
Genetic position: 83 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..522 401434..401955 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001195
References cited on this page View Complete Literature Guide for SPO12
1) Klapholz S and Esposito RE  (1980) Isolation of SPO12-1 and SPO13-1 from a natural variant of yeast that undergoes a single meiotic division. Genetics 96(3):567-88
2) Parkes V and Johnston LH  (1992) SPO12 and SIT4 suppress mutations in DBF2, which encodes a cell cycle protein kinase that is periodically expressed. Nucleic Acids Res 20(21):5617-23
3) Shah R, et al.  (2001) The Spo12 protein of Saccharomyces cerevisiae: a regulator of mitotic exit whose cell cycle-dependent degradation is mediated by the anaphase-promoting complex. Genetics 159(3):965-80
4) Stegmeier F, et al.  (2002) Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. Cell 108(2):207-20
5) Stern BM  (2003) FEARless in meiosis. Mol Cell 11(5):1123-5
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Esposito MS, et al.  (1970) Conditional mutants of meiosis in yeast. J Bacteriol 104(1):202-10