MSH1/YHR120W Summary Help

Standard Name MSH1 1
Systematic Name YHR120W
Feature Type ORF, Verified
Description DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis (1, 2, 3 and see Summary Paragraph)
Name Description MutS Homolog 1
Chromosomal Location
ChrVIII:349574 to 352453 | ORF Map | GBrowse
Genetic position: 70.44 cM
Gene Ontology Annotations All MSH1 GO evidence and references
  View Computational GO annotations for MSH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
52 total interaction(s) for 49 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 1
  • Two-hybrid: 10

Genetic Interactions
  • Negative Genetic: 14
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 3
  • Positive Genetic: 2
  • Synthetic Lethality: 1

Expression Summary
Length (a.a.) 959
Molecular Weight (Da) 109,407
Isoelectric Point (pI) 9.13
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:349574 to 352453 | ORF Map | GBrowse
Genetic position: 70.44 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2880 349574..352453 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001162

MSH1 is one of six MutS homologs in S. cerevisiae involved in mismatch repair but it is the only member of the family to function in the repair and maintenance of mitochondrial DNA (1, 4, 5). MSH1 is required for mitochondrial functioning and maintenance of mitochondrial morphology (4, 6). Msh1p has DNA-dependent ATPase activity and preferentially binds DNA substrates containing nucleotide mismatches and unpaired nucleotides (7, 2). Although MSH1 expression is increased during meiosis (3), there is no evidence that MSH1 is involved in meiotic recombination (8).

Msh1p shares 29% sequence identity with the bacterial MutS (1). The apparent lack of a MSH1 homolog in humans or other eukaryotes has led to the proposal that the high mutation rate of human mtDNA is due to limited mismatch repair (9).

Last updated: 2006-08-17 Contact SGD

References cited on this page View Complete Literature Guide for MSH1
1) Reenan RA and Kolodner RD  (1992) Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins. Genetics 132(4):963-73
2) Chi NW and Kolodner RD  (1994) The effect of DNA mismatches on the ATPase activity of MSH1, a protein in yeast mitochondria that recognizes DNA mismatches. J Biol Chem 269(47):29993-7
3) Meyer C, et al.  (2001) Transcription of mutS- and mutL-homologous genes during meiosis in Saccharomyces cerevisiae and identification of a regulatory cis-element for meiotic induction of MSH2. Mol Genet Genomics 265(5):826-36
4) Reenan RA and Kolodner RD  (1992) Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions. Genetics 132(4):975-85
5) Harfe BD and Jinks-Robertson S  (2000) Mismatch repair proteins and mitotic genome stability. Mutat Res 451(1-2):151-67
6) Mookerjee SA, et al.  (2005) Analysis of the functional domains of the mismatch repair homologue Msh1p and its role in mitochondrial genome maintenance. Curr Genet 47(2):84-99
7) Chi NW and Kolodner RD  (1994) Purification and characterization of MSH1, a yeast mitochondrial protein that binds to DNA mismatches. J Biol Chem 269(47):29984-92
8) Sia EA and Kirkpatrick DT  (2005) The yeast MSH1 gene is not involved in DNA repair or recombination during meiosis. DNA Repair (Amst) 4(2):253-61
9) Foury F, et al.  (2004) Mitochondrial DNA mutators. Cell Mol Life Sci 61(22):2799-811