MSH1/YHR120W Summary 
| Standard Name | MSH1 |
|---|---|
| Systematic Name | YHR120W |
| Feature Type | ORF, Verified |
| Description | DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis (1, 2, 3 and see Summary Paragraph) |
| Name Description | MutS Homolog 1 |
| Chromosomal Location | |
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| Genetic position: 70.44 cM |
Gene Ontology Annotations All MSH1 GO evidence and references
| View Computational GO annotations for MSH1 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated | |
| High-throughput |
Mutant phenotypes All MSH1 Phenotype evidence and references
interactions All MSH1 Interaction evidence and references
| 51 total interaction(s) for 48 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
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Expression Summary
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| Resources |
Protein Information All MSH1 Protein evidence and references
| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
sequence information
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| Genetic position: 70.44 cM | |||||||||||||
| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| Retrieve sequences | |||||||||||||
Analyze Sequence
| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
Resources
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000001162 |
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SUMMARY PARAGRAPH for MSH1
MSH1 is one of six MutS homologs in S. cerevisiae involved in mismatch repair but it is the only member of the family to function in the repair and maintenance of mitochondrial DNA (1, 4, 5). MSH1 is required for mitochondrial functioning and maintenance of mitochondrial morphology (4, 6). Msh1p has DNA-dependent ATPase activity and preferentially binds DNA substrates containing nucleotide mismatches and unpaired nucleotides (7, 2). Although MSH1 expression is increased during meiosis (3), there is no evidence that MSH1 is involved in meiotic recombination (8).
Msh1p shares 29% sequence identity with the bacterial MutS (1). The apparent lack of a MSH1 homolog in humans or other eukaryotes has led to the proposal that the high mutation rate of human mtDNA is due to limited mismatch repair (9).
Last updated: 2006-08-17 
References cited on this page View Complete Literature Guide for MSH1
| 1) | Reenan RA and Kolodner RD (1992) Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins. Genetics 132(4):963-73 |
| 2) | Chi NW and Kolodner RD (1994) The effect of DNA mismatches on the ATPase activity of MSH1, a protein in yeast mitochondria that recognizes DNA mismatches. J Biol Chem 269(47):29993-7 |
| 3) | Meyer C, et al. (2001) Transcription of mutS- and mutL-homologous genes during meiosis in Saccharomyces cerevisiae and identification of a regulatory cis-element for meiotic induction of MSH2. Mol Genet Genomics 265(5):826-36 |
| 4) | Reenan RA and Kolodner RD (1992) Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions. Genetics 132(4):975-85 |
| 5) | Harfe BD and Jinks-Robertson S (2000) Mismatch repair proteins and mitotic genome stability. Mutat Res 451(1-2):151-67 |
| 6) | Mookerjee SA, et al. (2005) Analysis of the functional domains of the mismatch repair homologue Msh1p and its role in mitochondrial genome maintenance. Curr Genet 47(2):84-99 |
| 7) | Chi NW and Kolodner RD (1994) Purification and characterization of MSH1, a yeast mitochondrial protein that binds to DNA mismatches. J Biol Chem 269(47):29984-92 |
| 8) | Sia EA and Kirkpatrick DT (2005) The yeast MSH1 gene is not involved in DNA repair or recombination during meiosis. DNA Repair (Amst) 4(2):253-61 |
| 9) | Foury F, et al. (2004) Mitochondrial DNA mutators. Cell Mol Life Sci 61(22):2799-811 |
