Standard Name	UBA4
Systematic Name	YHR111W
Alias	YHR1
Feature Type	ORF, Verified
Description	Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p (1, 2, 3, 4)
Name Description	UBiquitin-like protein Activating

Chromosomal Location	ChrVIII:333072 to 334394 | ORF Map | GBrowse

	View Computational GO annotations for UBA4
Molecular Function
Manually curated	protein adenylyltransferase activity (IMP) sulfurtransferase activity (IDA, IMP) thiosulfate sulfurtransferase activity (IDA) URM1 activating enzyme activity (IDA, IMP, ISS)
Biological Process
Manually curated	cell budding (IGI) cellular response to oxidative stress (IMP) invasive growth in response to glucose limitation (IGI, IMP) protein urmylation (IDA, IMP) regulation of pseudohyphal growth (IMP) tRNA thio-modification (IMP) tRNA wobble position uridine thiolation (IMP)
Cellular Component
High-throughput	cytoplasm (IDA)

Classical genetics
null	tRNA modification: absent tRNA-Glu modification: absent chronological lifespan: increased invasive growth: absent pseudohyphal growth: absent resistance to sirolimus: decreased resistance to canavanine: decreased spindle morphology: abnormal
reduction of function	tRNA-Glu modification: absent resistance to sirolimus: decreased
Large-scale survey
null	tRNA modification: abnormal auxotrophy competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased resistance to sirolimus: decreased resistance to 5-fluorouracil: decreased resistance to acetaldehyde: decreased resistance to oleate: decreased resistance to tunicamycin: increased resistance to mycophenolic acid: increased resistance to hydroxyurea: increased resistance to fenpropimorph: increased resistance to camptothecin: increased resistance to cycloheximide: decreased resistance to streptomycin: decreased resistance to bleomycin: decreased resistance to caffeine: decreased resistance to wortmannin: normal starvation resistance: decreased toxin resistance: decreased toxin resistance: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	212 total interaction(s) for 137 unique genes/features.
Physical Interactions	Affinity Capture-MS: 13 Affinity Capture-Western: 1 Biochemical Activity: 5 Reconstituted Complex: 1 Two-hybrid: 27
Genetic Interactions	Negative Genetic: 103 Phenotypic Enhancement: 1 Positive Genetic: 12 Synthetic Growth Defect: 41 Synthetic Lethality: 8
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVIII:333072 to 334394 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1323 333072..334394 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001153