RPF1/YHR088W Summary

RPF1 BASIC INFORMATION

Standard Name	RPF1
Systematic Name	YHR088W
Feature Type	ORF, Verified
Description	Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA (1, 2, 3, 4)

GO Annotations	All RPF1 GO evidence and references
	View Computational GO annotations for RPF1
Molecular Function
Manually curated	rRNA primary transcript binding (IPI)
Biological Process
Manually curated	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) ribosomal large subunit assembly (IPI) ribosomal large subunit export from nucleus (IMP)
Cellular Component
Manually curated	nucleolus (IDA, IPI) preribosome, large subunit precursor (IDA)
High-throughput	preribosome, large subunit precursor (IDA)

Mutant Phenotype	All RPF1 Phenotype details and references
Classical genetics
conditional	35S pre-rRNA accumulation: increased 27S rRNA processing intermediate accumulation: increased heat sensitivity: increased large ribosomal subunit distribution: abnormal
null	inviable
Large-scale survey
conditional	heat sensitivity: increased
null	inviable

Interactions	RPF1 All interactions details and references
	83 total interaction(s) for 51 unique genes/features.
Physical Interactions	Affinity Capture-MS: 71 Biochemical Activity: 1 Co-localization: 1 Reconstituted Complex: 4 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 1 Phenotypic Suppression: 1 Synthetic Lethality: 3

Sequence Information

ChrVIII:281497 to 282384 | |

Last Update

Coordinates: 2005-11-07 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..888	281497..282384	2005-11-07	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001130

RPF1 RESOURCES

Click on map for expanded view

SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for RPF1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RPF1]

1)	Wehner KA and Baserga SJ (2002) The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell 9(2):329-39
2)	Bogengruber E, et al. (2003) Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes. FEMS Yeast Res 3(1):35-43
3)	Horsey EW, et al. (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
4)	Caesar S, et al. (2006) Kap120 functions as a nuclear import receptor for ribosome assembly factor Rpf1 in yeast. Mol Cell Biol 26(8):3170-80

SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.