Standard Name	RPF1
Systematic Name	YHR088W
Feature Type	ORF, Verified
Description	Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA (1, 2, 3, 4)

Chromosomal Location	ChrVIII:281495 to 282382 | ORF Map | GBrowse

	View Computational GO annotations for RPF1
Molecular Function
Manually curated	rRNA primary transcript binding (IPI)
Biological Process
Manually curated	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) ribosomal large subunit assembly (IPI) ribosomal large subunit export from nucleus (IMP)
Cellular Component
Manually curated	nucleolus (IDA, IPI) preribosome, large subunit precursor (IDA)
High-throughput	preribosome, large subunit precursor (IDA)

Classical genetics
conditional	27S rRNA processing intermediate accumulation: increased 35S pre-rRNA accumulation: increased heat sensitivity: increased large ribosomal subunit distribution: abnormal
null	dessication resistance: increased inviable
Large-scale survey
conditional	chromosome/plasmid maintenance: abnormal colony sectoring: increased heat sensitivity: increased
null	inviable starvation resistance: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	114 total interaction(s) for 81 unique genes/features.
Physical Interactions	Affinity Capture-MS: 78 Affinity Capture-RNA: 2 Biochemical Activity: 1 Co-localization: 1 Reconstituted Complex: 6 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 20 Phenotypic Suppression: 1 Synthetic Growth Defect: 1 Synthetic Lethality: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVIII:281495 to 282382 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..888 281495..282382 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001130