NAM8/YHR086W Summary Help

Standard Name NAM8
Systematic Name YHR086W
Alias MRE2 , MUD15
Feature Type ORF, Verified
Description RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function (1, 2, 3, 4)
Name Description Nuclear Accommodation of Mitochondria
Chromosomal Location
ChrVIII:278153 to 279724 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NAM8 GO evidence and references
  View Computational GO annotations for NAM8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 124 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
214 total interaction(s) for 96 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 148
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Co-purification: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 31
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 8
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 523
Molecular Weight (Da) 56,972
Isoelectric Point (pI) 8.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:278153 to 279724 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1572 278153..279724 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001128
References cited on this page View Complete Literature Guide for NAM8
1) Nakagawa T and Ogawa H  (1997) Involvement of the MRE2 gene of yeast in formation of meiosis-specific double-strand breaks and crossover recombination through RNA splicing. Genes Cells 2(1):65-79
2) Puig O, et al.  (1999) Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection. Genes Dev 13(5):569-80
3) Ogawa H, et al.  (1995) Functions of the yeast meiotic recombination genes, MRE11 and MRE2. Adv Biophys 31:67-76
4) Qiu ZR, et al.  (2011) Defining the Mer1 and Nam8 meiotic splicing regulons by cDNA rescue. RNA 17(9):1648-54