STE12/YHR084W Summary Help

Standard Name STE12
Systematic Name YHR084W
Feature Type ORF, Verified
Description Transcription factor that is activated by a MAPK signaling cascade; activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth (1, 2 and see Summary Paragraph)
Name Description STErile
Chromosomal Location
ChrVIII:274174 to 276240 | ORF Map | GBrowse
Genetic position: 65.64 cM
Gene Ontology Annotations All STE12 GO evidence and references
  View Computational GO annotations for STE12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 176 genes
Regulators 5 genes
Classical genetics
Large-scale survey
172 total interaction(s) for 94 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 38
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 15
  • Biochemical Activity: 4
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • FRET: 1
  • PCA: 2
  • Reconstituted Complex: 17
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Rescue: 23
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 35
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 2
  • Synthetic Rescue: 9

Expression Summary
Length (a.a.) 688
Molecular Weight (Da) 77,866
Isoelectric Point (pI) 5.44
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:274174 to 276240 | ORF Map | GBrowse
Genetic position: 65.64 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2067 274174..276240 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001126

Yeast respond to mating pheromone by transducing the pheromone signal through a well-studied mitogen-activated protein kinase (MAPK) cascade (3). The culmination of this kinase cascade differentiates the cell for mating by activating transcription, altering cellular morphology, and arresting the cell in G1 phase. Phosphorylation by either of the two partially redundant kinases Fus3p and Kss1p at the end of the kinase cascade activates the Ste12 transcription factor, resulting in expression of genes involved in mating (4, 5). Ste12p binds to pheromone response elements (PREs) in the upstream activating sequences of its target genes (6, 7, 8). Ste12p also regulates genes involved in pseudohyphal growth in diploids and invasive growth in haploids (9, 2, 10). In addition to sharing a transcription factor, the mating and filamentous growth pathways also share many of the same kinases involved in the mating response (11). Developmental specificity to distinguish these pathways is determined by the requirement of another transcription factor, Tec1p, in the filamentous but not mating pathway (2, 10). Tec1p works in concert with Ste12p to cooperatively bind to filamentation response elements (FREs) in genes involved in filamentous growth (10).

Last updated: 1999-03-23 Contact SGD

References cited on this page View Complete Literature Guide for STE12
1) Bardwell L, et al.  (1998) Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK. Genes Dev 12(18):2887-98
2) Roberts RL and Fink GR  (1994) Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes Dev 8(24):2974-85
3) Herskowitz I  (1995) MAP kinase pathways in yeast: for mating and more. Cell 80(2):187-97
4) Elion EA, et al.  (1993) FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol Biol Cell 4(5):495-510
5) Elion EA, et al.  (1991) FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction. Proc Natl Acad Sci U S A 88(21):9392-6
6) Company M, et al.  (1988) Identification of a Ty1 regulatory sequence responsive to STE7 and STE12. Mol Cell Biol 8(6):2545-54
7) Errede B and Ammerer G  (1989) STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes. Genes Dev 3(9):1349-61
8) Hagen DC, et al.  (1991) Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. Mol Cell Biol 11(6):2952-61
9) Liu H, et al.  (1993) Elements of the yeast pheromone response pathway required for filamentous growth of diploids. Science 262(5140):1741-4
10) Madhani HD and Fink GR  (1997) Combinatorial control required for the specificity of yeast MAPK signaling. Science 275(5304):1314-7
11) Madhani HD, et al.  (1997) MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation. Cell 91(5):673-84
12) Dolan JW, et al.  (1989) The yeast STE12 protein binds to the DNA sequence mediating pheromone induction. Proc Natl Acad Sci U S A 86(15):5703-7
13) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
14) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87