STE12/YHR084W Summary Help

STE12 BASIC INFORMATION

Standard Name STE12
Systematic Name YHR084W
Feature Type ORF, Verified
Description Transcription factor that is activated by a MAP kinase signaling cascade, activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth (1, 2 and see Summary Paragraph)
Name Description STErile
GO Annotations All STE12 GO evidence and references
    View Computational GO annotations for STE12
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs STE12 Transcription Factor Binding Sites and References
  • ATGAAACA
  • TGAAAC
Regulatory modules predicted: stressResponse ( 367 )
Mutant Phenotype All STE12 Phenotype details and references
Classical genetics
conditional
null
overexpression
unspecified
Large-scale survey
null
overexpression
Interactions STE12 All interactions details and references
126 total interaction(s) for 74 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-Western: 11
  • Biochemical Activity: 4
  • Co-fractionation: 1
  • FRET: 1
  • PCA: 2
  • Reconstituted Complex: 13
  • Two-hybrid: 11
Genetic Interactions
  • Dosage Rescue: 24
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 21
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1
  • Synthetic Rescue: 9
Sequence Information
ChrVIII:274176 to 276242 | ORF Map | GBrowse
Gbrowse
Genetic position: 65.64 cM
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2067 274176..276242 2005-11-07 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001126

STE12 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for STE12

SUMMARY PARAGRAPH for STE12

Yeast respond to mating pheromone by transducing the pheromone signal through a well-studied mitogen-activated protein kinase (MAPK) cascade (3). The culmination of this kinase cascade differentiates the cell for mating by activating transcription, altering cellular morphology, and arresting the cell in G1 phase. Phosphorylation by either of the two partially redundant kinases Fus3p and Kss1p at the end of the kinase cascade activates the Ste12 transcription factor, resulting in expression of genes involved in mating (4, 5). Ste12p binds to pheromone response elements (PREs) in the upstream activating sequences of its target genes (6, 7, 8). Ste12p also regulates genes involved in pseudohyphal growth in diploids and invasive growth in haploids (9, 2, 10). In addition to sharing a transcription factor, the mating and filamentous growth pathways also share many of the same kinases involved in the mating response (11). Developmental specificity to distinguish these pathways is determined by the requirement of another transcription factor, Tec1p, in the filamentous but not mating pathway (2, 10). Tec1p works in concert with Ste12p to cooperatively bind to filamentation response elements (FREs) in genes involved in filamentous growth (10).

Last updated: 1999-03-23

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for STE12]

1) Bardwell L, et al.  (1998) Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK. Genes Dev 12(18):2887-98
2) Roberts RL and Fink GR  (1994) Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes Dev 8(24):2974-85
3) Herskowitz I  (1995) MAP kinase pathways in yeast: for mating and more. Cell 80(2):187-97
4) Elion EA, et al.  (1993) FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol Biol Cell 4(5):495-510
5) Elion EA, et al.  (1991) FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction. Proc Natl Acad Sci U S A 88(21):9392-6
6) Company M, et al.  (1988) Identification of a Ty1 regulatory sequence responsive to STE7 and STE12. Mol Cell Biol 8(6):2545-54
7) Errede B and Ammerer G  (1989) STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes. Genes Dev 3(9):1349-61
8) Hagen DC, et al.  (1991) Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. Mol Cell Biol 11(6):2952-61
9) Liu H, et al.  (1993) Elements of the yeast pheromone response pathway required for filamentous growth of diploids. Science 262(5140):1741-4
10) Madhani HD and Fink GR  (1997) Combinatorial control required for the specificity of yeast MAPK signaling. Science 275(5304):1314-7
11) Madhani HD, et al.  (1997) MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation. Cell 91(5):673-84