SSF1/YHR066W Summary

SSF1/YHR066W Summary

Standard Name	SSF1 1 (see Nomenclature conflict Note)
Systematic Name	YHR066W
Feature Type	ORF, Verified
Description	Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description	Suppressor of ste4 (Four) 1

Chromosomal Location	ChrVIII:229335 to 230696 \| ORF Map \| GBrowse

Gene Ontology Annotations All SSF1 GO evidence and references

	View Computational GO annotations for SSF1
Molecular Function
Manually curated	rRNA binding (IPI)
Biological Process
Manually curated	conjugation with cellular fusion (IGI) ribosomal large subunit assembly (IMP, IPI)
High-throughput	regulation of cell size (IMP)
Cellular Component
Manually curated	nucleolus (IDA)

Mutant phenotypes All SSF1 Phenotype evidence and references

Classical genetics
null	60S ribosomal subunit accumulation: decreased replicative lifespan: increased vegetative growth: decreased rate
Large-scale survey
null	cell size: decreased chronological lifespan: decreased competitive fitness: decreased fermentative growth: decreased rate heat sensitivity: increased replicative lifespan: increased resistance to cycloheximide: decreased resistance to bleomycin: increased resistance to cycloheximide: increased resistance to fluconazole: increased resistance to methyl methanesulfonate: increased resistance to mycophenolic acid: increased resistance to 5-fluorouracil: decreased resistance to arsenite(3-): decreased resistance to neomycin: decreased resistance to benzo[a]pyrene: decreased stress resistance: increased toxin resistance: increased transposable element transposition: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SSF1 Interaction evidence and references

	291 total interaction(s) for 194 unique genes/features.
Physical Interactions	Affinity Capture-MS: 84 Affinity Capture-Western: 33 PCA: 2 Two-hybrid: 1
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 4 Negative Genetic: 98 Phenotypic Enhancement: 6 Phenotypic Suppression: 1 Positive Genetic: 55 Synthetic Growth Defect: 3 Synthetic Lethality: 1 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SSF1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVIII:229335 to 230696 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1362	229335..230696	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001108

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
SPT16	Nomenclature conflict	SSF1 has been used in the literature to refer to both SPT16/YGL207W, which encodes a transcription elongation factor and SSF1/YHR066W, which encodes a rRNA binding protein.

References cited on this page View Complete Literature Guide for SSF1

1)	Yu Y and Hirsch JP (1995) An essential gene pair in Saccharomyces cerevisiae with a potential role in mating. DNA Cell Biol 14(5):411-8
2)	Fatica A, et al. (2002) Ssf1p prevents premature processing of an early pre-60S ribosomal particle. Mol Cell 9(2):341-51
3)	Bogengruber E, et al. (2003) Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes. FEMS Yeast Res 3(1):35-43
4)	Horsey EW, et al. (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61