ARG4/YHR018C Summary

Standard Name	ARG4
Systematic Name	YHR018C
Feature Type	ORF, Verified
Description	Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway (1 and see Summary Paragraph)
Name Description	ARGinine requiring

Chromosomal Location	ChrVIII:141402 to 140011 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: 12 cM

Gene Ontology Annotations All ARG4 GO evidence and references

	View Computational GO annotations for ARG4
Molecular Function
Manually curated	argininosuccinate lyase activity (IDA)
Biological Process
Manually curated	arginine biosynthetic process via ornithine (IDA)
Cellular Component
Manually curated	cytosol (IDA)

Pathways	arginine biosynthesis

Mutant phenotypes All ARG4 Phenotype evidence and references

Classical genetics
null	toxin resistance: increased
reduction of function	auxotrophy
Large-scale survey
null	arginine accumulation: decreased competitive fitness: decreased haploproficient resistance to farnesol: decreased stress resistance: decreased transposable element transposition: increased utilization of nitrogen source: absent vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ARG4 Interaction evidence and references

	11 total interaction(s) for 11 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 2 Two-hybrid: 1
Genetic Interactions	Dosage Lethality: 1 Synthetic Growth Defect: 1 Synthetic Lethality: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ARG4 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVIII:141402 to 140011 | |

Note: this feature is encoded on the Crick strand.

This feature contains embedded feature(s): ARS2

: 12 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1392	141402..140011	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001060

SUMMARY PARAGRAPH for ARG4

ARG4 encodes arginosuccinate lyase (1), a cytosolic enzyme that catalyzes the final step in arginine biosynthesis (2). Transcription of ARG4 is regulated by the general amino acid control mechanism (3, 4, 5); unlike other arginine biosynthetic genes, ARG4 is not regulated specifically in response to arginine (3). The ARG4 gene has been extensively used in studies of meiotic recombination and gene conversion; for reviews see 6 and 7.

Last updated: 1999-10-15

References cited on this page View Complete Literature Guide for ARG4

1)	Beacham IR, et al. (1984) The nucleotide sequence of the yeast ARG4 gene. Gene 29(3):271-9
2)	Jauniaux JC, et al. (1978) Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol 133(3):1096-1107
3)	Thiry-Blaise LM and Loppes R (1990) Deletion analysis of the ARG4 promoter of Saccharomyces cerevisiae: a poly(dAdT) stretch involved in gene transcription. Mol Gen Genet 223(3):474-80
4)	Messenguy F and Dubois E (1983) Participation of transcriptional and post-transcriptional regulatory mechanisms in the control of arginine metabolism in yeast. Mol Gen Genet 189(1):148-56
5)	Hinnebusch A (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6)	Strathern JN (1992) Recent writings on yeast recombination. Curr Opin Genet Dev 2(5):691-7
7)	Nicolas A and Petes TD (1994) Polarity of meiotic gene conversion in fungi: contrasting views. Experientia 50(3):242-52