ECM29/YHL030W Summary Help

Standard Name ECM29 1
Systematic Name YHL030W
Feature Type ORF, Verified
Description Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; inhibits proteasomal ATPase activity; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress (2, 3, 4)
Name Description ExtraCellular Mutant 1
Chromosomal Location
ChrVIII:40084 to 45690 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ECM29 GO evidence and references
  View Computational GO annotations for ECM29
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
unspecified
Resources
284 total interaction(s) for 188 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 224
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 13
  • Biochemical Activity: 1
  • Co-purification: 3
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 15
  • Phenotypic Suppression: 6
  • Positive Genetic: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 4
  • Synthetic Rescue: 10

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,868
Molecular Weight (Da) 210,429
Isoelectric Point (pI) 6.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:40084 to 45690 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..5607 40084..45690 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001022
References cited on this page View Complete Literature Guide for ECM29
1) Lussier M, et al.  (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2) Leggett DS, et al.  (2002) Multiple associated proteins regulate proteasome structure and function. Mol Cell 10(3):495-507
3) Lehmann A, et al.  (2010) Ecm29 fulfils quality control functions in proteasome assembly. Mol Cell 38(6):879-88
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76