OPI1/YHL020C Summary Help

Standard Name OPI1
Systematic Name YHL020C
Feature Type ORF, Verified
Description Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol (1, 2, 3, 4)
Name Description OverProducer of Inositol 5
Chromosomal Location
ChrVIII:67456 to 66242 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All OPI1 GO evidence and references
  View Computational GO annotations for OPI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs Predicted OPI1 Binding Site Locations
    Regulatory modules predicted: cellcycle (215)
    Resources
    Mutant phenotypes All OPI1 Phenotype evidence and references
    Classical genetics
    null
    unspecified
    Large-scale survey
    null
    Resources
    interactions All OPI1 Interaction evidence and references
    217 total interaction(s) for 170 unique genes/features.
    Physical Interactions
    • Affinity Capture-MS: 5
    • Affinity Capture-RNA: 2
    • Affinity Capture-Western: 7
    • Biochemical Activity: 5
    • Co-purification: 1
    • Reconstituted Complex: 3
    • Two-hybrid: 7
    Genetic Interactions
    • Dosage Rescue: 4
    • Negative Genetic: 139
    • Phenotypic Enhancement: 2
    • Positive Genetic: 16
    • Synthetic Growth Defect: 9
    • Synthetic Lethality: 4
    • Synthetic Rescue: 13
    Resources
    Expression Summary
    histogram
    Resources
    Protein Information All OPI1 Protein evidence and references
    Localization
    Phosphorylation PhosphoGRID | PhosphoPep Database
    Structure
    Homologs
    sequence information
    ChrVIII:67456 to 66242 | ORF Map | GBrowse
    Note: this feature is encoded on the Crick strand.
    SGD ORF map
    Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
    Subfeature details
    Relative
    Coordinates    		 Chromosomal
    Coordinates    		 Most Recent Updates
    Coordinates Sequence
    CDS 1..1215 67456..66242 2011-02-03 1996-07-31
    Retrieve sequences
    Analyze Sequence
    S288C only
    S288C vs. other species
    S288C vs. other strains
    Resources
    External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
    Primary SGDIDS000001012
    References cited on this page View Complete Literature Guide for OPI1
    1) Sreenivas A and Carman GM  (2003) Phosphorylation of the yeast phospholipid synthesis regulatory protein Opi1p by protein kinase A. J Biol Chem 278(23):20673-80
    2) Santiago TC and Mamoun CB  (2003) Genome expression analysis in yeast reveals novel transcriptional regulation by inositol and choline and new regulatory functions for Opi1p, Ino2p, and Ino4p. J Biol Chem 278(40):38723-30
    3) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
    4) Luevano-Martinez LA, et al.  (2013) Deletion of the transcriptional regulator opi1p decreases cardiolipin content and disrupts mitochondrial metabolism in Saccharomyces cerevisiae. Fungal Genet Biol ()
    5) Greenberg ML, et al.  (1982) Regulatory mutations of inositol biosynthesis in yeast: isolation of inositol-excreting mutants. Genetics 100(1):19-33
    6) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104