OPI1/YHL020C Summary Help

Standard Name OPI1 1
Systematic Name YHL020C
Feature Type ORF, Verified
Description Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol (2, 3, 4, 5)
Name Description OverProducer of Inositol 1
Chromosomal Location
ChrVIII:67456 to 66242 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All OPI1 GO evidence and references
  View Computational GO annotations for OPI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 47 genes
Regulators 5 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
repressible
Resources
221 total interaction(s) for 169 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Biochemical Activity: 5
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 139
  • Phenotypic Enhancement: 3
  • Positive Genetic: 17
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 4
  • Synthetic Rescue: 14

Resources
Expression Summary
histogram
Resources
Length (a.a.) 404
Molecular Weight (Da) 46,065
Isoelectric Point (pI) 4.87
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:67456 to 66242 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1215 67456..66242 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001012
References cited on this page View Complete Literature Guide for OPI1
1) Greenberg ML, et al.  (1982) Regulatory mutations of inositol biosynthesis in yeast: isolation of inositol-excreting mutants. Genetics 100(1):19-33
2) Sreenivas A and Carman GM  (2003) Phosphorylation of the yeast phospholipid synthesis regulatory protein Opi1p by protein kinase A. J Biol Chem 278(23):20673-80
3) Santiago TC and Mamoun CB  (2003) Genome expression analysis in yeast reveals novel transcriptional regulation by inositol and choline and new regulatory functions for Opi1p, Ino2p, and Ino4p. J Biol Chem 278(40):38723-30
4) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
5) Luevano-Martinez LA, et al.  (2013) Deletion of the transcriptional regulator opi1p decreases cardiolipin content and disrupts mitochondrial metabolism in Saccharomyces cerevisiae. Fungal Genet Biol 60():150-8
6) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104