PAB1/YER165W Summary

PAB1 BASIC INFORMATION

Standard Name	PAB1
Systematic Name	YER165W
Feature Type	ORF, Verified
Description	Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G (1, 2, 3)
Name Description	Poly(A) Binding protein 4

GO Annotations	All PAB1 GO evidence and references
	View Computational GO annotations for PAB1
Molecular Function
Manually curated	poly(A) RNA binding (IDA, IMP)
Biological Process
Manually curated	regulation of nuclear-transcribed mRNA poly(A) tail shortening (IDA, IMP) regulation of translational initiation (IDA, IMP)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA) ribosome (IDA) stress granule (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant Phenotype	All PAB1 Phenotype details and references
Classical genetics
null	inviable
reduction of function	heat sensitivity: increased
Large-scale survey
null	competitive fitness: decreased haploinsufficient inviable
repressible	mitochondrial morphology: abnormal

Interactions	PAB1 All interactions details and references
	208 total interaction(s) for 135 unique genes/features.
Physical Interactions	Affinity Capture-MS: 91 Affinity Capture-RNA: 1 Affinity Capture-Western: 23 Biochemical Activity: 4 Co-fractionation: 1 Co-localization: 1 Co-purification: 1 Reconstituted Complex: 17 Two-hybrid: 11
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 11 Phenotypic Enhancement: 20 Phenotypic Suppression: 9 Synthetic Growth Defect: 2 Synthetic Lethality: 5 Synthetic Rescue: 10

Sequence Information

ChrV:510368 to 512101 | |

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1734	510368..512101	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000967

PAB1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PAB1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PAB1]

1)	Kessler SH and Sachs AB (1998) RNA recognition motif 2 of yeast Pab1p is required for its functional interaction with eukaryotic translation initiation factor 4G. Mol Cell Biol 18(1):51-7
2)	Wyers F, et al. (2000) Deletion of the PAT1 gene affects translation initiation and suppresses a PAB1 gene deletion in yeast. Mol Cell Biol 20(10):3538-49
3)	Amrani N, et al. (1997) Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro. Mol Cell Biol 17(7):3694-701
4)	Sachs AB, et al. (1987) A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. Mol Cell Biol 7(9):3268-76

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