RAD4/YER162C Summary Help

Standard Name RAD4 1, 2
Systematic Name YER162C
Feature Type ORF, Verified
Description Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins (3, 4, 5, 6 and see Summary Paragraph)
Name Description RADiation sensitive
Chromosomal Location
ChrV:502894 to 500630 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 128 cM
Gene Ontology Annotations All RAD4 GO evidence and references
  View Computational GO annotations for RAD4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All RAD4 Phenotype evidence and references
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
Resources
interactions All RAD4 Interaction evidence and references
177 total interaction(s) for 131 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 18
  • Biochemical Activity: 1
  • Co-purification: 1
  • Two-hybrid: 9
Genetic Interactions
  • Negative Genetic: 87
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 1
  • Positive Genetic: 20
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1
Resources
Expression Summary
histogram
Resources
Protein Information All RAD4 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:502894 to 500630 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 128 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2265 502894..500630 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000964
SUMMARY PARAGRAPH for RAD4

RAD4 encodes a protein that acts in nucleotide excision repair (NER) of UV-damaged DNA. In NER, damaged DNA is excised by incision of the DNA on either side of the lesion followed by unwinding by DNA helicases; the process of NER is reviewed in 7. Rad4p and Rad23p form a stoichiometric complex called NEF2 that is required for the incision step of NER in an in vitro reconsituted reaction (8). NEF2 binds specifically to UV-damaged DNA (9, 10). Rad7p and Rad16p form another complex, called NEF4, that binds cooperatively with NEF2 to damaged DNA (11). The in vitro incision reaction is enhanced when both NEF2 and NEF4 are present (11). rad4 mutants are extremely sensitive to UV and completely defective in the incision step of NER (12).

In humans, NER deficiencies are associated with xeroderma pigmentosum (XP) and Cockayne syndrome (7, 13). RAD4 is homologous to the gene associated with XP-C (13); the human Rad4p homolog copurifies with a protein homologous to Rad23p (14). Rad4p homologs have also been found in several other eukaryotes, including S. pombe (15) and Drosophila (16).

Last updated: 1999-11-18

References cited on this page View Complete Literature Guide for RAD4
1) Morrison, D.P.  (1978) Repair parameters in mutator mutants of Saccharomyces cerevisiae. Ph.D Thesis
2) Sitney, K.  (1987) Genetic and molecular studies of the RAD24 gene of Saccharomyces. Ph.D. Thesis
3) Prakash S and Prakash L  (2000) Nucleotide excision repair in yeast. Mutat Res 451(1-2):13-24
4) Lommel L, et al.  (2002) Proteolysis of a nucleotide excision repair protein by the 26 S proteasome. Curr Genet 42(1):9-20
5) de Laat WL, et al.  (1999) Molecular mechanism of nucleotide excision repair. Genes Dev 13(7):768-85
6) Li Y, et al.  (2010) Rad4 regulates protein turnover at a postubiquitylation step. Mol Biol Cell 21(1):177-85
7) Friedberg EC, et al.  (1995) DNA Repair and Mutagenesis. Washington, DC: American Society for Microbiology (ASM) Press
8) Guzder SN, et al.  (1995) Reconstitution of yeast nucleotide excision repair with purified Rad proteins, replication protein A, and transcription factor TFIIH. J Biol Chem 270(22):12973-6
9) Guzder SN, et al.  (1998) Affinity of yeast nucleotide excision repair factor 2, consisting of the Rad4 and Rad23 proteins, for ultraviolet damaged DNA. J Biol Chem 273(47):31541-6
10) Jansen LE, et al.  (1998) Preferential binding of yeast Rad4.Rad23 complex to damaged DNA. J Biol Chem 273(50):33111-4
11) Guzder SN, et al.  (1999) Synergistic interaction between yeast nucleotide excision repair factors NEF2 and NEF4 in the binding of ultraviolet-damaged DNA. J Biol Chem 274(34):24257-62
12) Bankmann M, et al.  (1992) Yeast RAD14 and human xeroderma pigmentosum group A DNA-repair genes encode homologous proteins. Nature 355(6360):555-8
13) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10
14) Masutani C, et al.  (1994) Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23. EMBO J 13(8):1831-43
15) Choi IS, et al.  (1991) A gene in Schizosaccharomyces pombe analogous to the RAD4 gene of Saccharomyces cerevisiae. FEMS Microbiol Lett 61(1):97-100
16) Henning KA, et al.  (1994) Cloning the Drosophila homolog of the xeroderma pigmentosum complementation group C gene reveals homology between the predicted human and Drosophila polypeptides and that encoded by the yeast RAD4 gene. Nucleic Acids Res 22(3):257-61