Standard Name	SPT2 1
Systematic Name	YER161C
Alias	SIN1 , EXA1
Feature Type	ORF, Verified
Description	Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins (2, 3)
Name Description	SuPpressor of Ty's 1

Chromosomal Location	ChrV:500348 to 499347 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 127 cM

	View Computational GO annotations for SPT2
Molecular Function
Manually curated	DNA binding (IDA) DNA secondary structure binding (IDA)
Biological Process
Manually curated	chromatin organization (IMP) negative regulation of transcription from RNA polymerase II promoter (IMP) RNA polyadenylation (IGI, IMP) transcription elongation from RNA polymerase II promoter (IGI)
Cellular Component
Manually curated	nucleus (IDA)

Binding motifs	Predicted SPT2 Binding Site Locations
	YMTGTMTYTAW 4 [Search intergenic regions for YMTGTMTYTAW]
Resources	ScerTF | YEASTRACT | YeTFaSCo

Classical genetics
null	chromosome segregation: abnormal chromosome/plasmid maintenance: abnormal mitotic recombination: increased
overexpression	cell cycle progression in S phase: arrested chromosome/plasmid maintenance: abnormal
reduction of function	SSA3 mRNA accumulation: increased
Large-scale survey
null	competitive fitness: decreased resistance to caffeine: increased resistance to fenpropimorph: increased resistance to mycophenolic acid: increased resistance to tunicamycin: increased resistance to bleomycin: decreased resistance to sirolimus: decreased starvation resistance: decreased stress resistance: decreased viable
overexpression	vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	250 total interaction(s) for 177 unique genes/features.
Physical Interactions	Affinity Capture-MS: 26 Affinity Capture-RNA: 2 Affinity Capture-Western: 4 Co-localization: 1 Reconstituted Complex: 3 Two-hybrid: 10
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Dosage Rescue: 7 Negative Genetic: 130 Phenotypic Enhancement: 5 Phenotypic Suppression: 6 Positive Genetic: 14 Synthetic Growth Defect: 13 Synthetic Lethality: 8 Synthetic Rescue: 19
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:500348 to 499347 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 127 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1002 500348..499347 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000963