SPT2/YER161C Summary Help

Standard Name SPT2 1
Systematic Name YER161C
Alias SIN1 , EXA1
Feature Type ORF, Verified
Description Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components; relocalizes to the cytosol in response to hypoxia; similar to mammalian HMG1 proteins (2, 3, 4)
Name Description SuPpressor of Ty's 1
Chromosomal Location
ChrV:500348 to 499347 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 127 cM
Gene Ontology Annotations All SPT2 GO evidence and references
  View Computational GO annotations for SPT2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 79 genes
Regulators 1 genes
Resources
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
overexpression
Resources
270 total interaction(s) for 184 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 31
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 9
  • Biochemical Activity: 4
  • Co-localization: 5
  • Reconstituted Complex: 3
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 7
  • Negative Genetic: 131
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 6
  • Positive Genetic: 15
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 8
  • Synthetic Rescue: 19

Resources
Expression Summary
histogram
Resources
Length (a.a.) 333
Molecular Weight (Da) 38,551
Isoelectric Point (pI) 10.22
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:500348 to 499347 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 127 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1002 500348..499347 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000963
References cited on this page View Complete Literature Guide for SPT2
1) Winston F, et al.  (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
2) Perez-Martin J and Johnson AD  (1998) The C-terminal domain of Sin1 interacts with the SWI-SNF complex in yeast. Mol Cell Biol 18(7):4157-64
3) Hershkovits G, et al.  (2006) Recruitment of mRNA cleavage/polyadenylation machinery by the yeast chromatin protein Sin1p/Spt2p. Proc Natl Acad Sci U S A 103(26):9808-13
4) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
5) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104