SPI1/YER150W Summary Help

Standard Name SPI1 1
Systematic Name YER150W
Feature Type ORF, Verified
Description GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; SPI1 has a paralog, SED1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Stationary Phase Induced 1
Chromosomal Location
ChrV:468370 to 468816 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SPI1 GO evidence and references
  View Computational GO annotations for SPI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 32 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
27 total interaction(s) for 21 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • Co-purification: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 21
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 148
Molecular Weight (Da) 14,996
Isoelectric Point (pI) 4.02
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:468370 to 468816 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..447 468370..468816 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000952
References cited on this page View Complete Literature Guide for SPI1
1) Puig S and Perez-Ortin JE  (2000) Stress response and expression patterns in wine fermentations of yeast genes induced at the diauxic shift. Yeast 16(2):139-48
2) Hamada K, et al.  (1998) Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae. Mol Gen Genet 258(1-2):53-9
3) Kapteyn JC, et al.  (2001) Low external pH induces HOG1-dependent changes in the organization of the Saccharomyces cerevisiae cell wall. Mol Microbiol 39(2):469-79
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Simoes T, et al.  (2006) The SPI1 Gene, Encoding a Glycosylphosphatidylinositol-Anchored Cell Wall Protein, Plays a Prominent Role in the Development of Yeast Resistance to Lipophilic Weak-Acid Food Preservatives. Appl Environ Microbiol 72(11):7168-75