DDI1/YER143W Summary Help

Standard Name DDI1 1
Systematic Name YER143W
Alias VSM1
Feature Type ORF, Verified
Description DNA damage-inducible v-SNARE binding protein; role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains (2, 3, 4, 5)
Name Description DNA Damage Inducible 1
Chromosomal Location
ChrV:456319 to 457605 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DDI1 GO evidence and references
  View Computational GO annotations for DDI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
89 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 28
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 3
  • Negative Genetic: 14
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 428
Molecular Weight (Da) 47,354
Isoelectric Point (pI) 4.8
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:456319 to 457605 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1287 456319..457605 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000945
References cited on this page View Complete Literature Guide for DDI1
1) Liu Y and Xiao W  (1997) Bidirectional regulation of two DNA-damage-inducible genes, MAG1 and DDI1, from Saccharomyces cerevisiae. Mol Microbiol 23(4):777-89
2) Liu Y, et al.  (1997) UAS(MAG1), a yeast cis-acting element that regulates the expression of MAG1, is located within the protein coding region of DDI1. Mol Gen Genet 255(5):533-42
3) Lustgarten V and Gerst JE  (1999) Yeast VSM1 encodes a v-SNARE binding protein that may act as a negative regulator of constitutive exocytosis. Mol Cell Biol 19(6):4480-94
4) Clarke DJ, et al.  (2001) Dosage suppressors of pds1 implicate ubiquitin-associated domains in checkpoint control. Mol Cell Biol 21(6):1997-2007
5) White RE, et al.  (2011) The retroviral proteinase active site and the N-terminus of Ddi1 are required for repression of protein secretion. FEBS Lett 585(1):139-42