VFA1/YER128W Summary Help

Standard Name VFA1 1
Systematic Name YER128W
Feature Type ORF, Verified
Description Protein that interacts with Vps4p and has a role in vacuolar sorting; stimulates the ATPase activity of Vps4; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology (1, 2, 3)
Name Description Vps Four-Associated 1
Chromosomal Location
ChrV:415859 to 416470 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VFA1 GO evidence and references
  View Computational GO annotations for VFA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
overexpression
Large-scale survey
null
Resources
25 total interaction(s) for 23 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 12

Genetic Interactions
  • Negative Genetic: 7
  • Positive Genetic: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 203
Molecular Weight (Da) 23,488
Isoelectric Point (pI) 8.72
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:415859 to 416470 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..612 415859..416470 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000930
References cited on this page View Complete Literature Guide for VFA1
1) Arlt H, et al.  (2011) An overexpression screen in Saccharomyces cerevisiae identifies novel genes that affect endocytic protein trafficking. Traffic 12(11):1592-603
2) SGD  (2006) No information available
3) Vild CJ and Xu Z  (2014) Vfa1 Binds to the N-terminal Microtubule-interacting and Trafficking (MIT) Domain of Vps4 and Stimulates Its ATPase Activity. J Biol Chem 289(15):10378-86