GLO3/YER122C Summary Help

Standard Name GLO3
Systematic Name YER122C
Feature Type ORF, Verified
Description ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p (1, 2 and see Summary Paragraph)
Name Description GLyOxalase
Chromosomal Location
ChrV:404352 to 402871 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All GLO3 GO evidence and references
  View Computational GO annotations for GLO3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
419 total interaction(s) for 250 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 11
  • Biochemical Activity: 24
  • Co-purification: 1
  • PCA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 16
  • Negative Genetic: 151
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 137
  • Positive Genetic: 10
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 23
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 493
Molecular Weight (Da) 55,093
Isoelectric Point (pI) 8.46
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:404352 to 402871 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1482 404352..402871 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000924

GLO3 encodes a GTPase activating protein (GAP) for ADP ribosylation factors (ARFs, encoded by ARF1, ARF2, and ARF3 in yeast), and interacts genetically with ARF1 and ARF2 (3). ARFs are GTPases of the Ras superfamily that regulate the formation of coated vesicles in intracellular trafficking (4, 5). The process of vesicle formation in the exocytic and endocytic pathways has been recently reviewed (6), as has the role of ARF (7). GCS1 encodes an ARF GAP with sequence similarity to Glo3p; a gcs1 glo3 double deletion is inviable (3). Genetic interactions with BET1, BOS1, and SEC22, which encode v-SNARES, implicate Gcs1p and Glo3p in transport between the ER and the Golgi (3).

Last updated: 1999-10-29 Contact SGD

References cited on this page View Complete Literature Guide for GLO3
1) Dogic D, et al.  (1999) The ADP-ribosylation factor GTPase-activating protein Glo3p is involved in ER retrieval. Eur J Cell Biol 78(5):305-10
2) Zhang CJ, et al.  (2003) Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions. Yeast 20(4):315-30
3) Poon PP, et al.  (1999) Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function. EMBO J 18(3):555-64
4) Moss J and Vaughan M  (1998) Molecules in the ARF orbit. J Biol Chem 273(34):21431-4
5) Moss J and Vaughan M  (1999) Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol Cell Biochem 193(1-2):153-7
6) Springer S, et al.  (1999) A primer on vesicle budding. Cell 97(2):145-8
7) Roth MG  (1999) Snapshots of ARF1: implications for mechanisms of activation and inactivation. Cell 97(2):149-52