GLO3/YER122C Summary

Standard Name	GLO3
Systematic Name	YER122C
Feature Type	ORF, Verified
Description	ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p (1, 2 and see Summary Paragraph)
Name Description	GLyOxalase

Chromosomal Location	ChrV:404352 to 402871 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All GLO3 GO evidence and references

	View Computational GO annotations for GLO3
Molecular Function
Manually curated	ARF GTPase activator activity (IDA)
Biological Process
Manually curated	COPI coating of Golgi vesicle (IDA, IMP) ER to Golgi vesicle-mediated transport (IGI, IMP) retrograde vesicle-mediated transport, Golgi to ER (IDA, IGI, IMP)
Cellular Component
Manually curated	COPI vesicle coat (IPI) COPI-coated vesicle (IDA) endoplasmic reticulum-Golgi intermediate compartment (IPI)

Mutant phenotypes All GLO3 Phenotype evidence and references

Classical genetics
null	cold sensitivity: increased vegetative growth: decreased rate
Large-scale survey
null	alkaline pH resistance: decreased auxotrophy cell size: decreased rubidium(1+) accumulation: decreased glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased mitochondrial morphology: abnormal resistance to trichothecene: increased resistance to nickel(2+): decreased resistance to ethanol: decreased resistance to 4-chlorophenol: decreased resistance to clioquinol: decreased resistance to 2,4-dichlorophenol: decreased resistance to L-1,4-dithiothreitol: decreased resistance to 1,4-dithiothreitol: decreased resistance to acrolein: decreased resistance to hygromycin B: decreased respiratory growth: decreased rate respiratory growth: absent vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GLO3 Interaction evidence and references

	297 total interaction(s) for 162 unique genes/features.
Physical Interactions	Affinity Capture-MS: 15 Affinity Capture-RNA: 2 Affinity Capture-Western: 11 Biochemical Activity: 24 Co-purification: 1 PCA: 1 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 16 Negative Genetic: 41 Phenotypic Enhancement: 8 Phenotypic Suppression: 137 Positive Genetic: 1 Synthetic Growth Defect: 11 Synthetic Lethality: 23 Synthetic Rescue: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GLO3 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:404352 to 402871 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1482	404352..402871	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000924

SUMMARY PARAGRAPH for GLO3

GLO3 encodes a GTPase activating protein (GAP) for ADP ribosylation factors (ARFs, encoded by ARF1, ARF2, and ARF3 in yeast), and interacts genetically with ARF1 and ARF2 (3). ARFs are GTPases of the Ras superfamily that regulate the formation of coated vesicles in intracellular trafficking (4, 5). The process of vesicle formation in the exocytic and endocytic pathways has been recently reviewed (6), as has the role of ARF (7). GCS1 encodes an ARF GAP with sequence similarity to Glo3p; a gcs1 glo3 double deletion is inviable (3). Genetic interactions with BET1, BOS1, and SEC22, which encode v-SNARES, implicate Gcs1p and Glo3p in transport between the ER and the Golgi (3).

Last updated: 1999-10-29

References cited on this page View Complete Literature Guide for GLO3

1)	Dogic D, et al. (1999) The ADP-ribosylation factor GTPase-activating protein Glo3p is involved in ER retrieval. Eur J Cell Biol 78(5):305-10
2)	Zhang CJ, et al. (2003) Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions. Yeast 20(4):315-30
3)	Poon PP, et al. (1999) Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function. EMBO J 18(3):555-64
4)	Moss J and Vaughan M (1998) Molecules in the ARF orbit. J Biol Chem 273(34):21431-4
5)	Moss J and Vaughan M (1999) Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol Cell Biochem 193(1-2):153-7
6)	Springer S, et al. (1999) A primer on vesicle budding. Cell 97(2):145-8
7)	Roth MG (1999) Snapshots of ARF1: implications for mechanisms of activation and inactivation. Cell 97(2):149-52