SHO1/YER118C Summary Help

Standard Name SHO1
Systematic Name YER118C
Alias SSU81
Feature Type ORF, Verified
Description Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p (1, 2, 3, 4, 5, 6, 7)
Chromosomal Location
ChrV:399055 to 397952 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SHO1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
210 total interaction(s) for 135 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 17
  • Biochemical Activity: 1
  • PCA: 29
  • Protein-peptide: 1
  • Reconstituted Complex: 11
  • Two-hybrid: 52

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 63
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 9
  • Positive Genetic: 1
  • Synthetic Growth Defect: 8
  • Synthetic Haploinsufficiency: 2
  • Synthetic Lethality: 5
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 367
Molecular Weight (Da) 41,125
Isoelectric Point (pI) 5.44
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:399055 to 397952 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1104 399055..397952 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000920
References cited on this page View Complete Literature Guide for SHO1
1) Posas F and Saito H  (1997) Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: scaffold role of Pbs2p MAPKK. Science 276(5319):1702-5
2) Zarrinpar A, et al.  (2003) Optimization of specificity in a cellular protein interaction network by negative selection. Nature 426(6967):676-80
3) O'Rourke SM and Herskowitz I  (2004) Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis. Mol Biol Cell 15(2):532-42
4) Cullen PJ, et al.  (2004) A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast. Genes Dev 18(14):1695-708
5) Tatebayashi K, et al.  (2006) Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway. EMBO J 25(13):3033-44
6) Hao N, et al.  (2007) A systems-biology analysis of feedback inhibition in the Sho1 osmotic-stress-response pathway. Curr Biol 17(8):659-67
7) Tatebayashi K, et al.  (2007) Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway. EMBO J 26(15):3521-33