SLX8/YER116C Summary

SLX8 BASIC INFORMATION

Standard Name	SLX8 1
Systematic Name	YER116C
Feature Type	ORF, Verified
Description	Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain (1, 2, 3, 4)
Name Description	Synthetic Lethal of unknown (X) function 1

GO Annotations	All SLX8 GO evidence and references
	View Computational GO annotations for SLX8
Molecular Function
Manually curated	contributes_to ubiquitin-protein ligase activity (IDA) ubiquitin-protein ligase activity (IDA)
Biological Process
Manually curated	protein sumoylation (IGI, IMP) protein ubiquitination (IDA) response to DNA damage stimulus (IGI, IMP) telomere maintenance (IMP)
Cellular Component
Manually curated	nucleus (IDA) ubiquitin ligase complex (IDA)

Mutant Phenotype	All SLX8 Phenotype details and references
Classical genetics
null	UV resistance: decreased chromosome/plasmid maintenance: abnormal colony sectoring: increased Sumoylated-Mot1p accumulation: increased resistance to hydroxyurea: decreased resistance to canavanine: decreased
reduction of function	resistance to hydroxyurea: decreased
Large-scale survey
null	UV resistance: decreased glycogen accumulation: decreased competitive fitness: decreased mitotic recombination: increased Rad52-YFP distribution: increased resistance to hydroxyurea: decreased resistance to hygromycin B: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to doxorubicin: decreased sporulation: absent telomere length: increased viable

Interactions	SLX8 All interactions details and references
	298 total interaction(s) for 194 unique genes/features.
Physical Interactions	Affinity Capture-MS: 10 Affinity Capture-Western: 3 Biochemical Activity: 6 Co-purification: 1 Reconstituted Complex: 1 Two-hybrid: 6
Genetic Interactions	Phenotypic Enhancement: 103 Phenotypic Suppression: 40 Synthetic Growth Defect: 86 Synthetic Lethality: 36 Synthetic Rescue: 6

Sequence Information

ChrV:396168 to 395344 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..825	396168..395344	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000918

SLX8 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SLX8

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SLX8]

1)	Mullen JR, et al. (2001) Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae. Genetics 157(1):103-18
2)	Wang Z, et al. (2006) Genetic Analysis Connects SLX5 and SLX8 to the SUMO Pathway in Saccharomyces cerevisiae. Genetics 172(3):1499-509
3)	Xie Y, et al. (2007) The Yeast Hex3{middle dot}Slx8 Heterodimer Is a Ubiquitin Ligase Stimulated by Substrate Sumoylation. J Biol Chem 282(47):34176-84
4)	Ii T, et al. (2007) The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity. Cell Cycle 6(22):2800-9

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