BOI2/YER114C Summary Help

Standard Name BOI2 1, 2
Systematic Name YER114C
Alias BEB1
Feature Type ORF, Verified
Description Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication (3, 4)
Name Description Bem1 (One) Interacting protein 1, 2
Chromosomal Location
ChrV:393712 to 390590 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All BOI2 GO evidence and references
  View Computational GO annotations for BOI2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 82 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
118 total interaction(s) for 71 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Protein-peptide: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 31

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 43
  • Phenotypic Enhancement: 3
  • Positive Genetic: 3
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,040
Molecular Weight (Da) 115,687
Isoelectric Point (pI) 9.53
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:393712 to 390590 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3123 393712..390590 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000916
References cited on this page View Complete Literature Guide for BOI2
1) Matsui Y, et al.  (1996) Yeast src homology region 3 domain-binding proteins involved in bud formation. J Cell Biol 133(4):865-78
2) Bender L, et al.  (1996) Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast. J Cell Biol 133(4):879-94
3) Hallett MA, et al.  (2002) Probing the importance and potential roles of the binding of the PH-domain protein Boi1 to acidic phospholipids. BMC Cell Biol 3():16
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61