GLE2/YER107C Summary Help

Standard Name GLE2 1
Systematic Name YER107C
Alias RAE1
Feature Type ORF, Verified
Description RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 (1, 2, 3, 4 and see Summary Paragraph)
Name Description GLFG LEthal 1
Chromosomal Location
ChrV:374545 to 373448 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All GLE2 GO evidence and references
  View Computational GO annotations for GLE2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
111 total interaction(s) for 74 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 40
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 47
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 9

Expression Summary
Length (a.a.) 365
Molecular Weight (Da) 40,522
Isoelectric Point (pI) 8.49
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:374545 to 373448 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1098 374545..373448 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000909

GLE2 encodes a nuclear pore protein homologous to the nucleoporin Rae1p identified in S. pombe and mammalian cells (5, 1, 6, 7). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 7, 8, 9, 10). The structure of the vertebrate NPC has been studied extensively; recent reviews include 11, 12, 13, and 14. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (15, 16). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 7, 8, 17, 18, 19). GLE2 was identified by a mutation that is synthetically lethal with a nup100 null mutation (1), and interacts genetically with genes encoding other nucleoporins and nuclear transport factors (20). Gle2p forms a stable complex with the nucleoporin Nup116p (21, 22); binding to Nup116p is required for Gle2p to associate with nuclear pores (22). Gle2p also associates with the nuclear localization signal receptor Srp1p and Nup42p, which interacts with nuclear export signals. Mutations in GLE2 block export of polyadenylated RNA from the nucleus, and cause defects in NPC and nuclear envelope structure (1).

Last updated: 1999-08-05 Contact SGD

References cited on this page View Complete Literature Guide for GLE2
1) Murphy R, et al.  (1996) GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function. Mol Biol Cell 7(12):1921-37
2) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
3) Izawa S, et al.  (2004) Gle2p is essential to induce adaptation of the export of bulk poly(A)+ mRNA to heat shock in Saccharomyces cerevisiae. J Biol Chem 279(34):35469-78
4) Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
5) Kraemer D and Blobel G  (1997) mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm. Proc Natl Acad Sci U S A 94(17):9119-24
6) Pritchard CE, et al.  (1999) RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains. J Cell Biol 145(2):237-54
7) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
8) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
9) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
10) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
11) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
12) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
13) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
14) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
15) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
16) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
17) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
18) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
19) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
20) Ho AK, et al.  (1998) The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function. Mol Biol Cell 9(2):355-73
21) Iovine MK and Wente SR  (1997) A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p. J Cell Biol 137(4):797-811
22) Bailer SM, et al.  (1998) Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p. EMBO J 17(4):1107-19