VTC1/YER072W Summary Help

Standard Name VTC1 1
Systematic Name YER072W
Alias NRF1 2 , PHM4 3
Feature Type ORF, Verified
Description Subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress (1, 3, 4, 5, 6, 7, 8, 9)
Name Description Vacuolar Transporter Chaperone 1
Chromosomal Location
ChrV:302806 to 303195 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VTC1 GO evidence and references
  View Computational GO annotations for VTC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 10 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
109 total interaction(s) for 92 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 3
  • Biochemical Activity: 3
  • PCA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 69
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 7
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 129
Molecular Weight (Da) 14,371
Isoelectric Point (pI) 11.31
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:302806 to 303195 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..390 302806..303195 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000874
References cited on this page View Complete Literature Guide for VTC1
1) Cohen A, et al.  (1999) A novel family of yeast chaperons involved in the distribution of V-ATPase and other membrane proteins. J Biol Chem 274(38):26885-93
2) Murray JM and Johnson DI  (2000) Isolation and characterization of Nrf1p, a novel negative regulator of the Cdc42p GTPase in Schizosaccharomyces pombe. Genetics 154(1):155-65
3) Ogawa N, et al.  (2000) New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis. Mol Biol Cell 11(12):4309-21
4) Muller O, et al.  (2002) The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation. EMBO J 21(3):259-69
5) Muller O, et al.  (2003) Role of the Vtc proteins in V-ATPase stability and membrane trafficking. J Cell Sci 116(Pt 6):1107-15
6) Uttenweiler A, et al.  (2007) The vacuolar transporter chaperone (VTC) complex is required for microautophagy. Mol Biol Cell 18(1):166-75
7) Hothorn M, et al.  (2009) Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase. Science 324(5926):513-6
8) Tsvetanova NG, et al.  (2010) Proteome-Wide Search Reveals Unexpected RNA-Binding Proteins in Saccharomyces cerevisiae.LID - e12671 [pii] PLoS One 5(9)
9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76