MOT2/YER068W Summary Help

Standard Name MOT2
Systematic Name YER068W
Alias NOT4 , SIG1
Feature Type ORF, Verified
Description Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications (1, 2, 3, 4, 5, 6)
Name Description Modulator Of Transcription 7
Chromosomal Location
ChrV:293050 to 294813 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MOT2 GO evidence and references
  View Computational GO annotations for MOT2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs Predicted MOT2 Binding Site Locations
Resources
Mutant phenotypes All MOT2 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
interactions All MOT2 Interaction evidence and references
264 total interaction(s) for 166 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 34
  • Biochemical Activity: 3
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 14
  • Reconstituted Complex: 12
  • Two-hybrid: 13
Genetic Interactions
  • Dosage Growth Defect: 6
  • Dosage Lethality: 1
  • Dosage Rescue: 6
  • Negative Genetic: 32
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 4
  • Positive Genetic: 15
  • Synthetic Growth Defect: 37
  • Synthetic Lethality: 48
  • Synthetic Rescue: 5
Resources
Expression Summary
histogram
Resources
Protein Information All MOT2 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:293050 to 294813 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1764 293050..294813 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000870
References cited on this page View Complete Literature Guide for MOT2
1) Bai Y, et al.  (1999) The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5. Mol Cell Biol 19(10):6642-51
2) Liu HY, et al.  (1998) The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively. EMBO J 17(4):1096-106
3) Badarinarayana V, et al.  (2000) Functional interaction of CCR4-NOT proteins with TATAA-binding protein (TBP) and its associated factors in yeast. Genetics 155(3):1045-54
4) Denis CL, et al.  (2001) Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation. Genetics 158(2):627-34
5) Panasenko O, et al.  (2006) The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex. J Biol Chem 281(42):31389-98
6) Mersman DP, et al.  (2009) Polyubiquitination of the demethylase Jhd2 controls histone methylation and gene expression. Genes Dev 23(8):951-62
7) Cade RM and Errede B  (1994) MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae. Mol Cell Biol 14(5):3139-49
8) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
9) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87