MOT2/YER068W Summary Help

MOT2 BASIC INFORMATION

Standard Name MOT2
Systematic Name YER068W
Alias NOT4 , SIG1
Feature Type ORF, Verified
Description Subunit of the CCR4-NOT complex, which has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p (1, 2, 3, 4, 5, 6)
Name Description Modulator Of Transcription 7
GO Annotations All MOT2 GO evidence and references
    View Computational GO annotations for MOT2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs MOT2 Transcription Factor Binding Sites and References
  • ARGMAWCRAMAA
Mutant Phenotype All MOT2 Phenotype details and references
Classical genetics
null
reduction of function
Large-scale survey
null
Interactions MOT2 All interactions details and references
184 total interaction(s) for 126 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 25
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 14
  • Two-hybrid: 7
Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 6
  • Phenotypic Enhancement: 29
  • Phenotypic Suppression: 16
  • Synthetic Growth Defect: 29
  • Synthetic Lethality: 43
  • Synthetic Rescue: 4
Sequence Information
ChrV:293048 to 294811 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1764 293048..294811 1996-07-31 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000870

MOT2 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for MOT2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MOT2]

1) Bai Y, et al.  (1999) The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5. Mol Cell Biol 19(10):6642-51
2) Liu HY, et al.  (1998) The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively. EMBO J 17(4):1096-106
3) Badarinarayana V, et al.  (2000) Functional interaction of CCR4-NOT proteins with TATAA-binding protein (TBP) and its associated factors in yeast. Genetics 155(3):1045-54
4) Denis CL, et al.  (2001) Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation. Genetics 158(2):627-34
5) Panasenko O, et al.  (2006) The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex. J Biol Chem 281(42):31389-98
6) Mersman DP, et al.  (2009) Polyubiquitination of the demethylase Jhd2 controls histone methylation and gene expression. Genes Dev 23(8):951-62
7) Cade RM and Errede B  (1994) MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae. Mol Cell Biol 14(5):3139-49