YPT31/YER031C Summary

Standard Name	YPT31 1
Systematic Name	YER031C
Alias	YPT8 2
Feature Type	ORF, Verified
Description	Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication (1, 3, 4, 5, 6 and see Summary Paragraph)
Name Description	Yeast Protein Two 1

Chromosomal Location	ChrV:214747 to 214076 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All YPT31 GO evidence and references

	View Computational GO annotations for YPT31
Molecular Function
Manually curated	GTPase activity (IDA, IPI, ISS)
Biological Process
Manually curated	early endosome to Golgi transport (IGI) exocytosis (IMP) vesicle-mediated transport (IGI, IMP)
Cellular Component
Manually curated	endosome (IDA) Golgi apparatus (IDA)
High-throughput	mitochondrial outer membrane (IDA)

Mutant phenotypes All YPT31 Phenotype evidence and references

Classical genetics
null	Bud8p-GFP distribution: abnormal
Large-scale survey
null	budding pattern: abnormal cell shape: abnormal competitive fitness: decreased dessication resistance: decreased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to clioquinol: decreased resistance to mefloquine: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All YPT31 Interaction evidence and references

	288 total interaction(s) for 218 unique genes/features.
Physical Interactions	Affinity Capture-MS: 16 Affinity Capture-RNA: 3 Affinity Capture-Western: 8 Biochemical Activity: 1 Co-crystal Structure: 1 Co-purification: 2 PCA: 3 Reconstituted Complex: 4 Two-hybrid: 18
Genetic Interactions	Dosage Growth Defect: 3 Dosage Lethality: 3 Dosage Rescue: 22 Negative Genetic: 170 Phenotypic Enhancement: 3 Phenotypic Suppression: 2 Positive Genetic: 9 Synthetic Growth Defect: 9 Synthetic Lethality: 10 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All YPT31 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:214747 to 214076 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..672	214747..214076	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000833

SUMMARY PARAGRAPH for YPT31

Rab proteins are small Ras-related GTPases that function in multiple stages in membrane traffic. Rabs continuously cycle between the cytosol and membranes. The GDP-bound form of the Rab is complexed with in the cytosol and are recruited to the membranes with the help of GDF (GDI Displacement Factor) and by their prenylation by geranylgeranyl transferase II (GGTase II). The membrane-bound Rab is activated to the GTP-bound form by the guanine nucleotide exchange factors (GEFs) which then goes on to interact with downstream effectors. The GTPase-activating protein (GAP) stimulates the hydrolysis of GTP to GDP to inactivate the Rab. Eleven Rabs have been identified in yeast and more than 60 in mammalian cells (reviewed in 7, 8, 9).

Last updated: 2010-05-18

References cited on this page View Complete Literature Guide for YPT31

1)	Benli M, et al. (1996) Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast. EMBO J 15(23):6460-75
2)	Lai MH, et al. (1994) Identification of a gene encoding a new Ypt/Rab-like monomeric G-protein in Saccharomyces cerevisiae. Yeast 10(3):399-402
3)	Jedd G, et al. (1997) Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment. J Cell Biol 137(3):563-80
4)	Lazar T, et al. (1997) Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem Sci 22(12):468-72
5)	Wang W and Ferro-Novick S (2002) A Ypt32p exchange factor is a putative effector of Ypt1p. Mol Biol Cell 13(9):3336-43
6)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7)	Cai H, et al. (2007) Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12(5):671-82
8)	Grosshans BL, et al. (2006) Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci U S A 103(32):11821-7
9)	Bialek-Wyrzykowska U, et al. (2000) Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance. Mol Microbiol 35(6):1295-311