Standard Name	SRB4 1
Systematic Name	YER022W
Alias	MED17 2
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination (1, 3, 4)
Name Description	Suppressor of RNA polymerase B 1

Chromosomal Location	ChrV:198812 to 200875 | ORF Map | GBrowse

	View Computational GO annotations for SRB4
Molecular Function
Manually curated	activating transcription factor binding (IDA) core RNA polymerase II recruiting transcription factor activity (IMP, IPI) structural molecule activity (IMP)
Biological Process
Manually curated	positive regulation of transcription from RNA polymerase II promoter (IDA) transcription initiation from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	core mediator complex (IDA)

Classical genetics
conditional	poly(A)+ mRNA accumulation: decreased heat sensitivity: increased
Large-scale survey
null	haploinsufficient inviable starvation resistance: decreased toxin resistance: increased toxin resistance: decreased
overexpression	vegetative growth: decreased rate
reduction of function	competitive fitness: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	321 total interaction(s) for 97 unique genes/features.
Physical Interactions	Affinity Capture-MS: 127 Affinity Capture-RNA: 1 Affinity Capture-Western: 65 Co-localization: 2 Co-purification: 50 PCA: 1 Reconstituted Complex: 14 Two-hybrid: 13
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 2 Negative Genetic: 22 Phenotypic Enhancement: 4 Positive Genetic: 4 Synthetic Lethality: 3 Synthetic Rescue: 12
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:198812 to 200875 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2064 198812..200875 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000824