RPN3/YER021W Summary

RPN3 BASIC INFORMATION

Standard Name	RPN3 1
Systematic Name	YER021W
Alias	SUN2 2
Feature Type	ORF, Verified
Description	Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control (2, 3, 4, 5)
Name Description	Regulatory Particle Non-ATPase 1

GO Annotations	All RPN3 GO evidence and references
	View Computational GO annotations for RPN3
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	ubiquitin-dependent protein catabolic process (TAS)
Cellular Component
Manually curated	proteasome regulatory particle, lid subcomplex (IPI)

Mutant Phenotype	All RPN3 Phenotype details and references
Large-scale survey
null	inviable resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased

Interactions	RPN3 All interactions details and references
	200 total interaction(s) for 86 unique genes/features.
Physical Interactions	Affinity Capture-MS: 114 Affinity Capture-RNA: 1 Affinity Capture-Western: 10 Co-purification: 6 PCA: 6 Reconstituted Complex: 1 Two-hybrid: 6
Genetic Interactions	Dosage Rescue: 3 Phenotypic Enhancement: 1 Synthetic Growth Defect: 38 Synthetic Lethality: 1 Synthetic Rescue: 13

Sequence Information

ChrV:196947 to 198518 | |

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1572	196947..198518	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000823

RPN3 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for RPN3

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RPN3]

1)	Finley D, et al. (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2)	Kominami K, et al. (1997) Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1. Mol Biol Cell 8(1):171-87
3)	Glickman MH, et al. (1998) A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3. Cell 94(5):615-23
4)	Bailly E and Reed SI (1999) Functional characterization of rpn3 uncovers a distinct 19S proteasomal subunit requirement for ubiquitin-dependent proteolysis of cell cycle regulatory proteins in budding yeast. Mol Cell Biol 19(10):6872-90
5)	Kaiser P, et al. (1999) Cyclin-dependent kinase and Cks/Suc1 interact with the proteasome in yeast to control proteolysis of M-phase targets. Genes Dev 13(9):1190-202

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