RPN3/YER021W Summary Help

Standard Name RPN3 1
Systematic Name YER021W
Alias SUN2 2
Feature Type ORF, Verified
Description Essential non-ATPase regulatory subunit of the 26S proteasome lid; similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control (2, 3, 4, 5)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrV:196948 to 198519 | ORF Map | GBrowse
Gene Ontology Annotations All RPN3 GO evidence and references
  View Computational GO annotations for RPN3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Large-scale survey
reduction of function
244 total interaction(s) for 114 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 142
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 15
  • Co-purification: 6
  • PCA: 6
  • Reconstituted Complex: 5
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 42
  • Phenotypic Enhancement: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 5
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 523
Molecular Weight (Da) 60,392
Isoelectric Point (pI) 5.37
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:196948 to 198519 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1572 196948..198519 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000823
References cited on this page View Complete Literature Guide for RPN3
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Kominami K, et al.  (1997) Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1. Mol Biol Cell 8(1):171-87
3) Glickman MH, et al.  (1998) A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3. Cell 94(5):615-23
4) Bailly E and Reed SI  (1999) Functional characterization of rpn3 uncovers a distinct 19S proteasomal subunit requirement for ubiquitin-dependent proteolysis of cell cycle regulatory proteins in budding yeast. Mol Cell Biol 19(10):6872-90
5) Kaiser P, et al.  (1999) Cyclin-dependent kinase and Cks/Suc1 interact with the proteasome in yeast to control proteolysis of M-phase targets. Genes Dev 13(9):1190-202