GPA2/YER020W Summary Help

GPA2 BASIC INFORMATION

Standard Name GPA2 1
Systematic Name YER020W
Alias SSP101
Feature Type ORF, Verified
Description Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery (2, 3, 4, 5)
Name Description G Protein Alpha subunit 6
GO Annotations All GPA2 GO evidence and references
    View Computational GO annotations for GPA2
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All GPA2 Phenotype details and references
Classical genetics
activation
dominant negative
null
Large-scale survey
null
Interactions GPA2 All interactions details and references
89 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-Western: 9
  • PCA: 31
  • Protein-RNA: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 11
Genetic Interactions
  • Dosage Rescue: 6
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 8
  • Synthetic Rescue: 4
Sequence Information
ChrV:195167 to 196516 | ORF Map | GBrowse
Gbrowse
Genetic position: 10 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1350 195167..196516 1996-07-31 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000822

GPA2 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Literature
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  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for GPA2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GPA2]

1) Nakafuku M, et al.  (1988) Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein: studies on its structure and possible functions. Proc Natl Acad Sci U S A 85(5):1374-8
2) Colombo S, et al.  (1998) Involvement of distinct G-proteins, Gpa2 and Ras, in glucose- and intracellular acidification-induced cAMP signalling in the yeast Saccharomyces cerevisiae. EMBO J 17(12):3326-41
3) Donzeau M and Bandlow W  (1999) The yeast trimeric guanine nucleotide-binding protein alpha subunit, Gpa2p, controls the meiosis-specific kinase Ime2p activity in response to nutrients. Mol Cell Biol 19(9):6110-9
4) Harashima T and Heitman J  (2002) The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits. Mol Cell 10(1):163-73
5) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
6) Nakafuku M, et al.  (1987) Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the alpha subunit of mammalian G proteins. Proc Natl Acad Sci U S A 84(8):2140-4