ISC1/YER019W Summary Help

Standard Name ISC1 1
Systematic Name YER019W
Feature Type ORF, Verified
Description Inositol phosphosphingolipid phospholipase C; mitochondrial membrane localized; hydrolyzes complex sphingolipids to produce ceramide; activates genes required for non-fermentable carbon source metabolism during the diauxic shift; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance (1, 2, 3, 4)
Name Description Inositol phosphoSphingolipid phospholipase C 1
Chromosomal Location
ChrV:192797 to 194230 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ISC1 GO evidence and references
  View Computational GO annotations for ISC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Pathways
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
226 total interaction(s) for 173 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • PCA: 1
  • Protein-RNA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 97
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 6
  • Positive Genetic: 64
  • Synthetic Growth Defect: 28
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 477
Molecular Weight (Da) 53,940
Isoelectric Point (pI) 7.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:192797 to 194230 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1434 192797..194230 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000821
References cited on this page View Complete Literature Guide for ISC1
1) Sawai H, et al.  (2000) Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae. J Biol Chem 275(50):39793-8
2) Betz C, et al.  (2002) ISC1-encoded inositol phosphosphingolipid phospholipase C is involved in Na+/Li+ halotolerance of Saccharomyces cerevisiae. Eur J Biochem 269(16):4033-9
3) Okamoto Y, et al.  (2002) Structural requirements for selective binding of ISC1 to anionic phospholipids. J Biol Chem 277(48):46470-7
4) Kitagaki H, et al.  (2009) ISC1-dependent Metabolic Adaptation Reveals an Indispensable Role for Mitochondria in Induction of Nuclear Genes during the Diauxic Shift in Saccharomyces cerevisiae. J Biol Chem 284(16):10818-30