SPC25/YER018C Summary Help

Standard Name SPC25
Systematic Name YER018C
Feature Type ORF, Verified
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p (1, 2, 3)
Name Description Spindle Pole Component
Chromosomal Location
ChrV:192624 to 191959 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SPC25 GO evidence and references
  View Computational GO annotations for SPC25
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
Resources
186 total interaction(s) for 113 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 16
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-localization: 1
  • Co-purification: 3
  • PCA: 3
  • Reconstituted Complex: 5
  • Two-hybrid: 113

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 1
  • Phenotypic Enhancement: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 221
Molecular Weight (Da) 25,244
Isoelectric Point (pI) 6.91
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:192624 to 191959 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..666 192624..191959 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000820
References cited on this page View Complete Literature Guide for SPC25
1) Wigge PA and Kilmartin JV  (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60
2) Janke C, et al.  (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91
3) He X, et al.  (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206