SPC25/YER018C Summary

SPC25 BASIC INFORMATION

Standard Name	SPC25
Systematic Name	YER018C
Feature Type	ORF, Verified
Description	Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering (1, 2, 3)
Name Description	Spindle Pole Component

GO Annotations	All SPC25 GO evidence and references
	View Computational GO annotations for SPC25
Molecular Function
Manually curated	structural constituent of cytoskeleton (IPI)
Biological Process
Manually curated	chromosome segregation (IGI, IMP) microtubule nucleation (IPI)
Cellular Component
Manually curated	condensed nuclear chromosome kinetochore (IDA, IMP, IPI) condensed nuclear chromosome, centromeric region (IGI, IPI) Ndc80 complex (IDA, IPI)

Mutant Phenotype	All SPC25 Phenotype details and references
Large-scale survey
null	inviable

Interactions	SPC25 All interactions details and references
	118 total interaction(s) for 68 unique genes/features.
Physical Interactions	Affinity Capture-MS: 15 Affinity Capture-RNA: 1 Affinity Capture-Western: 15 Co-crystal Structure: 1 Co-fractionation: 1 Co-purification: 1 Reconstituted Complex: 2 Two-hybrid: 73
Genetic Interactions	Dosage Rescue: 5 Synthetic Growth Defect: 1 Synthetic Lethality: 3

Sequence Information

ChrV:192623 to 191958 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..666	192623..191958	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000820

SPC25 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SPC25

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SPC25]

1)	Wigge PA and Kilmartin JV (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60
2)	Janke C, et al. (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91
3)	He X, et al. (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206

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