Standard Name	PRP22 1
Systematic Name	YER013W
Feature Type	ORF, Verified
Description	DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes (2, 3, 4, 5, 6)
Name Description	Pre-mRNA Processing 1

Chromosomal Location	ChrV:178841 to 182278 | ORF Map | GBrowse

	View Computational GO annotations for PRP22
Molecular Function
Manually curated	ATP-dependent RNA helicase activity (IDA) contributes_to second spliceosomal transesterification activity (IDA, IMP)
Biological Process
Manually curated	generation of catalytic spliceosome for second transesterification step (IDA, IMP) spliceosomal complex disassembly (IMP)
Cellular Component
Manually curated	U2-type catalytic step 2 spliceosome (IMP) U2-type post-spliceosomal complex (IMP)

Classical genetics
conditional	unspliced intron-containing RNA accumulation: abnormal heat sensitivity: increased
Large-scale survey
conditional	resistance to benomyl: decreased
null	filamentous growth: decreased invasive growth: absent inviable
reduction of function	competitive fitness: increased resistance to methyl methanesulfonate: decreased telomere length: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	211 total interaction(s) for 181 unique genes/features.
Physical Interactions	Affinity Capture-MS: 50 Affinity Capture-RNA: 1 Biochemical Activity: 1 Reconstituted Complex: 1 Two-hybrid: 8
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 116 Phenotypic Enhancement: 1 Positive Genetic: 19 Synthetic Growth Defect: 4 Synthetic Lethality: 3 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:178841 to 182278 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3438 178841..182278 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000815