PCM1/YEL058W Summary Help

Standard Name PCM1 1
Systematic Name YEL058W
Alias AGM1
Feature Type ORF, Verified
Description Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors (1, 2, 3 and see Summary Paragraph)
Name Description PhosphoaCetylglucosamine Mutase 1
Chromosomal Location
ChrV:43252 to 44925 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PCM1 GO evidence and references
  View Computational GO annotations for PCM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 4 genes
Resources
Pathways
Large-scale survey
null
reduction of function
unspecified
Resources
18 total interaction(s) for 14 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 2

Genetic Interactions
  • Phenotypic Suppression: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 557
Molecular Weight (Da) 62,066
Isoelectric Point (pI) 5.78
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:43252 to 44925 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1674 43252..44925 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000784
SUMMARY PARAGRAPH for PCM1

UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) is the source of the first two GlcNAc moieties added during N-linked glycosylation of proteins and provides GlcNAc for synthesis of GPI anchors. In yeast, it is synthesized from fructose-6-phosphate (F6P) by the consecutive action of Gfa1p, Gna1p, Pcm1p, and Qri1p, although a different pathway (4, 5) is used in bacteria. UDP-GlcNAc is also the building block from which chitin, a linear polymer of beta-1,4-N-acetylglucosamine, is constructed. Chitin is a component of the cell wall deposited as a ring around the neck of the growing bud during cell division. Bud scars, the remnants of the chitinous primary septum seen on the surface of mother cells after division, are the primary repository of chitin (reviewed in 6).

PCM1 encodes phosphoacetylglucosamine mutase (EC 5.4.2.3) (1), which catalyzes the isomerization of GlcNAc-6-P to GlcNAc-1-P. While the reaction is reversible, GlcNAc-1-P is a substrate for Qri1p, which synthesizes UDP-GlcNAc. PCM1 is an essential gene. After sporulation of heterozygous diploids, spores lacking Pcm1p survive for only a few generations, forming undivided strings of four to five cells (1, 3) containing little or no chitin (3).

Interestingly, Pcm1p has enough nonspecific hexosephosphate mutase activity that overexpression of PCM1 complements a pgm1 pgm2 double mutant, which lacks the phosphoglucomutases that isomerize glucose-6-phosphate to glucose-1-phosphate (1, 7). The human homolog of PCM1 is called both AGM1 (phosphoacetylglucosamine mutase, as in yeast) and PGM3 (phosphoglucomutase) (8). Both human (OMIM) and Candida albicans AGM1 complement deletion of PCM1 (2).

Last updated: 2005-07-01 Contact SGD

References cited on this page View Complete Literature Guide for PCM1
1) Hofmann M, et al.  (1994) Characterization of the essential yeast gene encoding N-acetylglucosamine-phosphate mutase. Eur J Biochem 221(2):741-7
2) Mio T, et al.  (2000) Functional cloning and mutational analysis of the human cDNA for phosphoacetylglucosamine mutase: identification of the amino acid residues essential for the catalysis. Biochim Biophys Acta 1492(2-3):369-76
3) Igual JC, et al.  (1997) A genetic screen reveals a role for the late G1-specific transcription factor Swi4p in diverse cellular functions including cytokinesis. J Cell Sci 110 ( Pt 14)():1647-54
4) Mengin-Lecreulx D and van Heijenoort J  (1993) Identification of the glmU gene encoding N-acetylglucosamine-1-phosphate uridyltransferase in Escherichia coli. J Bacteriol 175(19):6150-7
5) Mengin-Lecreulx D and van Heijenoort J  (1994) Copurification of glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase activities of Escherichia coli: characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pathway for UDP-N-acetylglucosamine synthesis. J Bacteriol 176(18):5788-95
6) Cid VJ, et al.  (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59(3):345-86
7) Boles E, et al.  (1994) A family of hexosephosphate mutases in Saccharomyces cerevisiae. Eur J Biochem 220(1):83-96
8) Pang H, et al.  (2002) Identification of human phosphoglucomutase 3 (PGM3) as N-acetylglucosamine-phosphate mutase (AGM1). Ann Hum Genet 66(Pt 2):139-44