RAD23/YEL037C Summary Help

Standard Name RAD23
Systematic Name YEL037C
Feature Type ORF, Verified
Description Protein with ubiquitin-like N terminus; subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description RADiation sensitive
Chromosomal Location
ChrV:82603 to 81407 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -26 cM
Gene Ontology Annotations All RAD23 GO evidence and references
  View Computational GO annotations for RAD23
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
unspecified
Resources
344 total interaction(s) for 187 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 58
  • Affinity Capture-Western: 71
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 6
  • Reconstituted Complex: 21
  • Two-hybrid: 26

Genetic Interactions
  • Dosage Growth Defect: 10
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 71
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 6
  • Positive Genetic: 14
  • Synthetic Growth Defect: 26
  • Synthetic Lethality: 8
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 398
Molecular Weight (Da) 42,366
Isoelectric Point (pI) 4.07
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:82603 to 81407 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -26 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1197 82603..81407 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000763
SUMMARY PARAGRAPH for RAD23

RAD23 encodes a protein that acts in nucleotide excision repair (NER) of UV-damaged DNA. In NER, damaged DNA is excised by incision of the DNA on either side of the lesion followed by unwinding by DNA helicases; the process of NER is reviewed in 6. Rad23p and Rad4p form a stoichiometric complex called NEF2 that is required for the incision step of NER in an in vitro reconsituted reaction (7). NEF2 binds specifically to UV-damaged DNA (8, 9). Rad7p and Rad16p form another complex, called NEF4, that binds cooperatively with NEF2 to damaged DNA (10). The in vitro incision reaction is enhanced when both NEF2 and NEF4 are present (10). Rad23p has an N-terminal ubiquitin-like domain that interacts physically with the 26S proteasome (11).

In humans, NER deficiencies are associated with xeroderma pigmentosum (XP) and Cockayne syndrome (6, 12). Human homologs of Rad23p and Rad4p copurify; the Rad4p homolog is encoded by the human XP-C gene (13, 12).

Last updated: 1999-11-18 Contact SGD

References cited on this page View Complete Literature Guide for RAD23
1) Prakash S and Prakash L  (2000) Nucleotide excision repair in yeast. Mutat Res 451(1-2):13-24
2) Lommel L, et al.  (2002) Proteolysis of a nucleotide excision repair protein by the 26 S proteasome. Curr Genet 42(1):9-20
3) de Laat WL, et al.  (1999) Molecular mechanism of nucleotide excision repair. Genes Dev 13(7):768-85
4) Wang S, et al.  (2009) N-terminal deletion of Peptide:N-glycanase results in enhanced deglycosylation activity. PLoS One 4(12):e8335
5) Li Y, et al.  (2010) Rad4 regulates protein turnover at a postubiquitylation step. Mol Biol Cell 21(1):177-85
6) Friedberg EC, et al.  (1995) DNA Repair and Mutagenesis. Washington, DC: American Society for Microbiology (ASM) Press
7) Guzder SN, et al.  (1995) Reconstitution of yeast nucleotide excision repair with purified Rad proteins, replication protein A, and transcription factor TFIIH. J Biol Chem 270(22):12973-6
8) Guzder SN, et al.  (1998) Affinity of yeast nucleotide excision repair factor 2, consisting of the Rad4 and Rad23 proteins, for ultraviolet damaged DNA. J Biol Chem 273(47):31541-6
9) Jansen LE, et al.  (1998) Preferential binding of yeast Rad4.Rad23 complex to damaged DNA. J Biol Chem 273(50):33111-4
10) Guzder SN, et al.  (1999) Synergistic interaction between yeast nucleotide excision repair factors NEF2 and NEF4 in the binding of ultraviolet-damaged DNA. J Biol Chem 274(34):24257-62
11) Schauber C, et al.  (1998) Rad23 links DNA repair to the ubiquitin/proteasome pathway. Nature 391(6668):715-8
12) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10
13) Masutani C, et al.  (1994) Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23. EMBO J 13(8):1831-43