SPF1/YEL031W Summary Help

Standard Name SPF1 1 (see Nomenclature conflict Note)
Systematic Name YEL031W
Alias COD1 2 , PIO1 3 , PER9 4
Feature Type ORF, Verified
Description P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 (1, 2, 5, 6)
Name Description Sensitivity to Pichia Farinosa killer toxin 1
Chromosomal Location
ChrV:90258 to 93905 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SPF1 GO evidence and references
  View Computational GO annotations for SPF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All SPF1 Phenotype evidence and references
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
interactions All SPF1 Interaction evidence and references
488 total interaction(s) for 267 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • PCA: 2
Genetic Interactions
  • Negative Genetic: 335
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 48
  • Positive Genetic: 45
  • Synthetic Growth Defect: 22
  • Synthetic Lethality: 21
  • Synthetic Rescue: 2
Resources
Expression Summary
histogram
Resources
Protein Information All SPF1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:90258 to 93905 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3648 90258..93905 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000757

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
COG4Nomenclature conflictBoth SPF1/YEL031W, which encodes an ATPase of the ER membrane, and COG4/YPR105C, which encodes a constituent of a Golgi transport complex, have been referred to as COD1.
References cited on this page View Complete Literature Guide for SPF1
1) Suzuki C and Shimma YI  (1999) P-type ATPase spf1 mutants show a novel resistance mechanism for the killer toxin SMKT. Mol Microbiol 32(4):813-23
2) Cronin SR, et al.  (2000) Regulation of HMG-CoA reductase degradation requires the P-type ATPase Cod1p/Spf1p. J Cell Biol 148(5):915-24
3) Tipper DJ and Harley CA  (2002) Yeast genes controlling responses to topogenic signals in a model transmembrane protein. Mol Biol Cell 13(4):1158-74
4) Vashist S, et al.  (2002) Two distinctly localized p-type ATPases collaborate to maintain organelle homeostasis required for glycoprotein processing and quality control. Mol Biol Cell 13(11):3955-66
5) Cronin SR, et al.  (2002) Cod1p/Spf1p is a P-type ATPase involved in ER function and Ca2+ homeostasis. J Cell Biol 157(6):1017-28
6) Katrin K, et al.  (2012) Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes. Mol Biol Cell 23(20):3927-35